Human GIP Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GIP in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 911205
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    GIP Peptide
    Tyr1-Asn39
    Accession # P09681
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
GIP in Human Stomach. GIP was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human GIP Monoclonal Antibody (Catalog # MAB8864) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GIP
Gastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide is a member of the secretin family of hormones. GIP, together with glucagon-like peptide-1 (GLP-1), belongs to the group of metabolic hormones called incretins that stimulate a decrease in blood glucose levels. GIP is derived from a 153-amino acid pro-protein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide.  Engagement of Gastric inhibitory polypeptide receptors (GIPR) by GIP on pancreatic beta cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose.

GIP (1-39) is an endogenous truncated form of GIP that has been shown to be more potent in stimulating insulin secretion from rat pancreatic islets.

  • Long Name:
    Gastric Inhibitory Polypeptide
  • Entrez Gene IDs:
    2695 (Human); 14607 (Mouse); 25040 (Rat)
  • Alternate Names:
    gastric inhibitory polypeptide; GIP; Glucose-dependent Insulinotropic Polypeptide; Incretin
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