Human GIP Antibody

Catalog # Availability Size / Price Qty
MAB8864
MAB8864-SP
GIP in Human Stomach.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human GIP Antibody Summary

Species Reactivity
Human
Specificity
Detects human GIP in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 911205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
GIP Peptide
Tyr1-Asn39
Accession # P09681
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry GIP antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

GIP in Human Stomach. GIP was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human GIP Monoclonal Antibody (Catalog # MAB8864) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIP

Gastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide is a member of the secretin family of hormones. GIP, together with glucagon-like peptide-1 (GLP-1), belongs to the group of metabolic hormones called incretins that stimulate a decrease in blood glucose levels. GIP is derived from a 153-amino acid pro-protein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide.  Engagement of Gastric inhibitory polypeptide receptors (GIPR) by GIP on pancreatic beta cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose.

GIP (1-39) is an endogenous truncated form of GIP that has been shown to be more potent in stimulating insulin secretion from rat pancreatic islets.

Long Name
Gastric Inhibitory Polypeptide
Entrez Gene IDs
2695 (Human); 14607 (Mouse); 25040 (Rat)
Alternate Names
gastric inhibitory polypeptide; GIP; Glucose-dependent Insulinotropic Polypeptide; Incretin

Product Datasheets

Citation for Human GIP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. GIP_HUMAN[22-51] is a new proatherogenic peptide identified by native plasma peptidomics
    Authors: T Masaki, Y Kodera, M Terasaki, K Fujimoto, T Hirano, M Shichiri
    Scientific Reports, 2021;11(1):14470.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human GIP Antibody
By Anonymous on 01/12/2022
Application: IHC Sample Tested: Stomach tissue Species: Human