Human GluR6/GRIK2 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # FAB11512AFP405

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1060307
View all GluR6/GRIK2 Primary Antibodies »

Product Specifications

Specificity

Detects recombinant human GluR6/GRIK2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human GluR6/GRIK2 Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GluR6/GRIK2

The Ionotropic Glutamate Receptors are a family of ligand-gated ion channels involved in regulating fast excitatory synaptic transmission in the central nervous system (CNS) (1, 2). The family consists of ~18 members which is divided into 3 groups based on the activating agonist: N-Methyl-D-Aspartate (NMDA), alpha -Amino-3-hydroxy-5-Methyl-4-isoxazole Propionic Acid (AMPA) and kainate (KA) (1, 2). KA receptors (KAR) are found in the pre- and postsynaptic membranes of the central nervous system, most prominently in the hippocampus, where they regulate synaptic transmission, neuronal excitability, and network activity (3). Glutamate receptor 6 (GluR6) (also Glutamate receptor ionotropic, kainate 2 or GluK2) along with GluR5, GluR7 and receptor subunits KA1 and KA2 belong to the KAR sub-group as these molecules are activated by kainic acid (1-3). Mature human GluR6 is a 3-transmembrane protein with a long (530 aa) N-terminal extracellular domain (ECD), a M2 domain involved in pore formation and a short C-terminal intracellular domain (4). The ECD of human GluR6 is highly conserved and shares 99% identity with both mouse and rat. Under physiological conditions, GluR6 forms either a homomeric or heteromeric receptor channel with GluR5 or KA1/2 to become functional (5, 6). Activation of KAR complexes result in Ca2+-dependent release of glutamatergic or GABAergic signals (7). The modulatory role of KARs make them attractive therapeutic candidates as glutamate excitotoxicity has been associated with multiple neurodegenerative and mental disorders. Misregulation of GluR6 has been suggested to be a factor in autism, Huntington disease and epilepsy (8). Additionally, GluR6 has been suggested to play a role in tumor suppression in Gastric cancer (9) while potentially displaying the opposite effect in urological cancers (10).

References

  1. Dingledine, R. et al. (1999) Pharmacol Rev. 51(1):7.
  2. Traynelis, S.F. et al. (2010) Pharmacol Rev. 62(3):405.
  3. Contractor, A. et al. (2011) Trends Neurosci. 34(3):154.
  4. Nanao, M.H. et al. (2005) PNAS 102(5):1708.
  5. Kumar, J. et al. (2011) Neuron. 71(2):319.
  6. Fisher, J.L. et al. (2011) J. Neurosci. 31(47):17113.
  7. Mathew, S.S. and Hablitz, J.J. (2008) Neuropharmacology. 55(1):106.
  8. Lerma, J. and Marques, J.M. (2013) Neuron. 80(2):292.
  9. Wu, C.S. et al. (2010) Int J Cancer. 126(11):2542.
  10. Inoue, R. et al. (2017) Oncotarget. 8(17):28826.

Long Name

Glutamate Receptor 6

Alternate Names

EAA4, GLR6, GluK2, GLUK6, MRT6

Entrez Gene IDs

2898 (Human); 14806 (Mouse); 54257 (Rat)

Gene Symbol

GRIK2

UniProt

Q13002 (Human)

Additional GluR6/GRIK2 Products

Product Documents for Human GluR6/GRIK2 Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human GluR6/GRIK2 Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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