Human GluR6/GRIK2 Antibody (1060307)

R&D Systems | Catalog # MAB11512

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1060307
View all GluR6/GRIK2 Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human GluR6/GRIK2 protein
Thr32-Asn549
Accession # Q13002

Specificity

Detects recombinant human GluR6/GRIK2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GluR6/GRIK2 Antibody (1060307)

Detection of Human GluR6/GRIK2 by Western Blot.

Western Blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human GluR6/GRIK2+GFP. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human GluR6/GRIK2 Monoclonal Antibody (Catalog # MAB11512) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GluR6/GRIK2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human GluR6/GRIK2 Antibody (1060307)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HEK293T human embryonic kidney cell line either mock transfected or transfected with human GluR6/GRIK2+GFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GluR6/GRIK2

The Ionotropic Glutamate Receptors are a family of ligand-gated ion channels involved in regulating fast excitatory synaptic transmission in the central nervous system (CNS) (1, 2). The family consists of ~18 members which is divided into 3 groups based on the activating agonist: N-Methyl-D-Aspartate (NMDA), alpha -Amino-3-hydroxy-5-Methyl-4-isoxazole Propionic Acid (AMPA) and kainate (KA) (1, 2). KA receptors (KAR) are found in the pre- and postsynaptic membranes of the central nervous system, most prominently in the hippocampus, where they regulate synaptic transmission, neuronal excitability, and network activity (3). Glutamate receptor 6 (GluR6) (also Glutamate receptor ionotropic, kainate 2 or GluK2) along with GluR5, GluR7 and receptor subunits KA1 and KA2 belong to the KAR sub-group as these molecules are activated by kainic acid (1-3). Mature human GluR6 is a 3-transmembrane protein with a long (530 aa) N-terminal extracellular domain (ECD), a M2 domain involved in pore formation and a short C-terminal intracellular domain (4). The ECD of human GluR6 is highly conserved and shares 99% identity with both mouse and rat. Under physiological conditions, GluR6 forms either a homomeric or heteromeric receptor channel with GluR5 or KA1/2 to become functional (5, 6). Activation of KAR complexes result in Ca2+-dependent release of glutamatergic or GABAergic signals (7). The modulatory role of KARs make them attractive therapeutic candidates as glutamate excitotoxicity has been associated with multiple neurodegenerative and mental disorders. Misregulation of GluR6 has been suggested to be a factor in autism, Huntington disease and epilepsy (8). Additionally, GluR6 has been suggested to play a role in tumor suppression in Gastric cancer (9) while potentially displaying the opposite effect in urological cancers (10).

References

  1. Dingledine, R. et al. (1999) Pharmacol Rev. 51(1):7.
  2. Traynelis, S.F. et al. (2010) Pharmacol Rev. 62(3):405.
  3. Contractor, A. et al. (2011) Trends Neurosci. 34(3):154.
  4. Nanao, M.H. et al. (2005) PNAS 102(5):1708.
  5. Kumar, J. et al. (2011) Neuron. 71(2):319.
  6. Fisher, J.L. et al. (2011) J. Neurosci. 31(47):17113.
  7. Mathew, S.S. and Hablitz, J.J. (2008) Neuropharmacology. 55(1):106.
  8. Lerma, J. and Marques, J.M. (2013) Neuron. 80(2):292.
  9. Wu, C.S. et al. (2010) Int J Cancer. 126(11):2542.
  10. Inoue, R. et al. (2017) Oncotarget. 8(17):28826.

Long Name

Glutamate Receptor 6

Alternate Names

EAA4, GLR6, GluK2, GLUK6, MRT6

Entrez Gene IDs

2898 (Human); 14806 (Mouse); 54257 (Rat)

Gene Symbol

GRIK2

UniProt

Q13002 (Human)

Additional GluR6/GRIK2 Products

Product Documents for Human GluR6/GRIK2 Antibody (1060307)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GluR6/GRIK2 Antibody (1060307)

For research use only

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