Human GM-CSF XL Luminex® Performance Assay

Name: Human GM-CSF XL Luminex® Performance Assay
Brand: R&D Systems
SKU: LUXLM215
Price: 210 USD
Availability: InStock

R&D Systems | Catalog # LUXLM215

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Product Documents

Luminex High Performance Assays

Human GM-CSF XL Luminex® Performance Assay is specifically designed for multiplex detection of human GM-CSF with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.

This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.

Build Now

Build Your Assay
Product Features
Performance Validation
Kit Contents
Precision and Accuracy
Which Luminex Assay is Right for Me
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Panel

Human XL Cytokine Panel

Assay Type

Magnetic bead-based multiplex assay for the Luminex® platform

Format

1 x 96-well microplate and magnetic antibody-coated beads

Species

Human

Analytes Detected

GM-CSF

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

View all GM-CSF Luminex Assays »

Build Your Assay

Make Selections Below to Configure Your Custom Luminex® Assay

Configured Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If overlapping bead regions occur in analyte selections, send your Luminex configuration code to our team for resolution.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on sample dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human GM-CSF with a panel of related analytes in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate
Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*

*Please allow additional time for international orders.

Performance Validation

R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

View Luminex High Performance Human XL validation data.

Base Kit Contents

Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**
High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Bead Set Contents

User-mixed antibody-coated Magnetic beads in individual vials

Recommended Sample Types: Cell culture supernates, serum, EDTA plasma, and heparin plasma

Components: Human GM-CSF Magnetic Microparticles (Part 898830) is supplied as a 100X concentrated stock (0.075 mL) with preservatives. Other Supplies Required: Magnetic Luminex® Performance Assay Human XL Discovery Base Kit (R&D Systems®, Catalog # LUXLM000).

Sensitivity: The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the mean MFI of twenty zero standard replicates and calculating the corresponding concentration. Six assays were evaluated, and the MDD of human GM-CSF ranged from 0.855-3.82 pg/mL. The mean MDD was 1.56 pg/mL.

Correlation: This assay has been correlated to the Quantikine® ELISA Kit.

Specificity: This assay recognizes natural and recombinant human GM-CSF.

Bead Region: 37

Precision

	Intra-Assay Precision		Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Two samples of known concentration were tested in thirty separate assays to assess precision between assays.
Sample	1	2	1	2
n	20	20	30	30
Mean (pg/mL)	197	1829	197	1,863
Standard deviation	6.62	63.3	29.6	139
CV (%)	36.0	3.4	14.9	47.1

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human GM-CSF were evaluated for recovery.
Cell culture supernate	118	106-132
Serum	97.7	89.6-109
EDTA plasma	100	95.1-106
Heparin plasma	97.6	91.3-104
Plaelet-poor EDTA	111	85.6-133
Platelet-poor Heparin	109	93.2-124

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma	Platelet-poor EDTA	Platelet-poor Heparin
Samples containing and/or spiked with human GM-CSF were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	97.8	103	100	102	102	102
1:2	Range (%)	91.4-103	100-106	97.0-106	100-105	95.8-109	98.8-105
1:4	Average % of Expected	92.6	99.0	95.9	97.1	102	102
1:4	Range (%)	85.1-98.9	95.5-101	88.6-105	93.2-103	93.7-109	92.0-117
1:8	Average % of Expected	93.4	102	98.3	99.5	110	100
1:8	Range (%)	89.8-98.3	97.5-104	93.3-107	94.2-106	97.5-124	96.7-106
1:16	Average % of Expected	93.8	99.7	96.0	95.0	104	93.7
1:16	Range (%)	90.6-97.4	92.9-105	87.1-104	91.9-102	96.8-114	88.0-101

Which Luminex Assay is Right for Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.

High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.

Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.

Additional Information About Luminex Assays

All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.

Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.

Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.

Scientific Data Images for Human GM-CSF XL Luminex® Performance Assay

GM-CSF XL Cytokine Luminex Panel Standard Curves

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GM-CSF

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.