Human GPR64 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7977P-100
Detection of GPR64  in NS0 Cell Line Transfected with Human GPR64 and eGFP by Flow  Cytometry.
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Human GPR64 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR64 transfectants in cell-based ELISA and Flow Cytometry.
Source
Monoclonal Mouse IgG2B Clone # 206420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GPR64
Leu38-Asn64, Glu68-Thr553
Accession # NP_001073328
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of GPR64  in NS0 Cell Line Transfected with Human GPR64 and eGFP by Flow  Cytometry. View Larger

Detection of GPR64 in NS0 Cell Line Transfected with Human GPR64 and eGFP by Flow Cytometry. NS0 cell line transfected with Human GPR64 and eGFP was stained with (A) Mouse Anti-Human GPR64 PE-conjugated Monoclonal Antibody (Catalog # FAB7977P) or (B) Mouse IgG2B PE-conjugated isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR64

GPR64 (G-Protein coupled Receptor 64; also HE6) is a 110 kDa (predicted) member of the B class of GCPRs. Within this class GPR64 belongs to a Large N-termini family-B 7-transmembrane (LNB-7TM) subclass of receptors (also known as adhesion-GCPRs). GPR64 has restricted expression, being found in stereocilia cell membranes of epididymal caput epithelial cells and, to a limited extent, on osteoblasts. The function of GPR64 is somewhat unclear, but in the epididymis, it may be involved in fluid transport. Mature human GPR64 is a 980 amino acid (aa) 7-TM glycoprotein (SwissProt Q8IZP9). It contains an extended extracellular N-teminus (aa 38-627), seven consecutive TM segments (aa 628-878) and a C-terminal cytoplasmic tail (aa 879-1010). The extended extracellular region possesses a juxtamembrane GPS domain (aa 567-618) that serves as a proteolytic cleavage site. Enzymatic activity here generates a 180 kDa soluble form that stays associated with a 40 kDa membrane-embedded fragment. Notably, isolation of the membrane fragment gives rise to oligomers that run at > 250 kDa in SDS-PAGE. There are multiple splice variants. The one used for immunization to generate this antibody contains a deletion of aa 88-101 (RefSeq NP_001073328). Four other splice forms show single block deletions of aa 65-67, 51-66, 52-75, and 906-956, respectively. Three others possesses aa substitutions; a 20 aa block for aa 52-101, and a common 12 aa block that can substitute for either aa 68-101 or aa 52-101. Over aa 38-64 and 68-553 of RefSeq NP_001073328, human and mouse GPR64 share 69% aa sequence identity.

Long Name
G Protein-coupled Receptor 64
Entrez Gene IDs
10149 (Human); 237175 (Mouse); 266735 (Rat)
Alternate Names
EDDM6; epididymis-specific (seven transmembrane family); G protein-coupled receptor 64; GPR64; HE6; MGC104454; MGC138738; TM7LN2

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