Intracellular Staining by Flow Cytometry
|Detection of Granzyme A in Human CD56+ Whole Blood Cells by Flow Cytometry. Human CD56+ whole blood cells were stained with Mouse Anti-Human Granzyme A APC‑conjugated Monoclonal Antibody (Catalog # IC29051A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.|
Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1‑26), a propeptide (residues 27‑28) and a mature chain (residues 29‑262) (2). The purified recombinant human Granzyme A consists of residues 26 to 262. After being activated by lysyl endopeptidase, it cleaves a thioester substrate.
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