Human Guanylyl Cyclase C/GUCY2C Alexa Fluor® 750-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2157S-100UG
R&D Systems Antibodies
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Human Guanylyl Cyclase C/GUCY2C Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Guanylyl Cyclase C/GUCY2C in direct ELISAs.
Source
Monoclonal Rabbit IgG Clone # 2543C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell HEK293-derived human Guanylyl Cyclase C/GUCY2C
Ser21-Gln430
Accession # P25092
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line Transfected with Human GUCY2C and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References
  1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
  3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
  4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
  5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
  6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  7. Gong, R. et al. (2011) Science. 333:1642.
  8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.
Entrez Gene IDs
2984 (Human); 14917 (Mouse); 25711 (Rat)
Alternate Names
DIAR6; EC 4.6.1; GCC; GC-C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylate Cyclase C; Guanylyl Cyclase C; GUC2C; GUC2CEC 4.6.1.2; GUCY2C; heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MUCIL; STA Receptor; StAR; STARSTA receptor

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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