Human Guanylyl Cyclase C/GUCY2C Antibody

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Detection of GUCY2C in HEK293 Human Cell Line Transfected with Human GUCY2C and eGFP by Flow Cytometry.
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Human Guanylyl Cyclase C/GUCY2C Antibody Summary

Species Reactivity
Detects human Guanylyl Cyclase C/GUCY2C in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2543C
Protein A or G purified from cell culture supernatant
Human embryonic kidney cell HEK293-derived human Guanylyl Cyclase C/GUCY2C
Accession # P25092
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GUCY2C antibody in HEK293 Human Cell Line Transfected with Human GUCY2C and eGFP antibody by Flow Cytometry. View Larger

Detection of GUCY2C in HEK293 Human Cell Line Transfected with Human GUCY2C and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GUCY2C or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human GUCY2C Monoclonal Antibody (Catalog # MAB2157) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Guanylyl Cyclase C/GUCY2C antibody in LS1034 Human Cell Line by Immunocytochemistry (ICC). View Larger

Guanylyl Cyclase C/GUCY2C in LS1034 Human Cell Line. Guanylyl Cyclase C/GUCY2C was detected in immersion fixed LS1034 human colorectal carcinoma cell line (positive) and HCT-116 human colorectal carcinoma cell line (negative) using Rabbit Anti-Human Guanylyl Cyclase C/GUCY2C Monoclonal Antibody (Catalog # MAB2157) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

  1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
  3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
  4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
  5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
  6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  7. Gong, R. et al. (2011) Science. 333:1642.
  8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.
Entrez Gene IDs
2984 (Human); 14917 (Mouse); 25711 (Rat)
Alternate Names
DIAR6; EC 4.6.1; GCC; GC-C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylate Cyclase C; Guanylyl Cyclase C; GUC2C; GUC2CEC; GUCY2C; heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MUCIL; STA Receptor; StAR; STARSTA receptor

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