Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2543C
View all Guanylyl Cyclase C/GUCY2C Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human Guanylyl Cyclase C/GUCY2C
Ser21-Gln430
Accession # P25092

Specificity

Detects human Guanylyl Cyclase C/GUCY2C in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Antibody

Detection of Guanylyl Cyclase C/GUCY2C in hGUCY2C eGFP/HEK293 tfx cells by Flow Cytometry.

HEK293 transfected with Human GUCY2C and eGFP were stained with eGFP and either (A) Rabbit Anti-Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Monoclonal Antibody (Catalog # FAB2157P) or (B) Rabbit IgG PE-conjugated Antibody (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 transfected with Human GUCY2C and eGFP 

Reviewed Applications

Read 1 review rated 5 using FAB2157P in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.005 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References

  1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
  3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
  4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
  5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
  6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  7. Gong, R. et al. (2011) Science. 333:1642.
  8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Alternate Names

DIAR6, GC-C, GCC, Guanylate Cyclase C, GUC2C, GUCY2C, MUCIL, STA Receptor, StAR

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Gene Symbol

GUCY2C

UniProt

P25092

Additional Guanylyl Cyclase C/GUCY2C Products

Product Documents for Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Antibody

For research use only

Citations for Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Antibody

Customer Reviews for Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Antibody (1)

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  • Human Guanylyl Cyclase C/GUCY2C Antibody
    Name: Panagiotis Fotakis
    Application: Flow Cytometry
    Sample Tested: T84 human colon carcinoma cell line
    Species: Human
    Verified Customer | Posted 02/18/2024
    Detection of human GUCY2C on T84 cancer cell line. The cells were treated with 5 ul of the GUCY2C-PE antibody(BLUE) or isotype control antibody (RED) for 30 minutes on ice.
    Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Antibody FAB2157P

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