Human HAI-1 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB1048R Datasheet / COA / SDS

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FAB1048R-100UG

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Human HAI-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HAI-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human HAI-2 or recombinant mouse HAI-1 is observed.

Source

Monoclonal Mouse IgG1 Clone # 169417

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Val449
Accession # O43278

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HAI-1

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

Long Name

HGF Activator Inhibitor Type 1

Entrez Gene IDs

6692 (Human); 20732 (Mouse)

Alternate Names

HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1