HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 169417
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Val449
Accession # O43278
Pro37-Val449
Accession # O43278
Specificity
Detects human HAI-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human HAI-2 or recombinant mouse HAI-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human HAI-1 Antibody (169417)
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human HAI‑1 (Catalog # 1048-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human HAI‑1 (Catalog # 1048-PI), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human HAI‑1 (Catalog # 1048-PI)
Sample: Recombinant Human HAI‑1 (Catalog # 1048-PI)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HAI-1
References
- Denda, K. et al. (2002) J. Biol. Chem. 277:14053.
- Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
- Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Alternate Names
HAI1, SPINT1
Gene Symbol
SPINT1
UniProt
Additional HAI-1 Products
Product Documents for Human HAI-1 Antibody (169417)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HAI-1 Antibody (169417)
For research use only
Related Research Areas
Customer Reviews for Human HAI-1 Antibody (169417)
There are currently no reviews for this product. Be the first to review Human HAI-1 Antibody (169417) and earn rewards!
Have you used Human HAI-1 Antibody (169417)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...