Human HE4/WFDC2 Quantikine ELISA Kit

R&D Systems | Catalog # DHE400

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (10 µL), Urine (10 µL)

Sensitivity

32.2 pg/mL

Assay Range

78.1-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
View all HE4/WFDC2 ELISA Kits »

Product Summary for Human HE4/WFDC2 Quantikine ELISA Kit

The Quantikine Human HE4/WFDC2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HE4/WFDC2 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human HE4/WFDC2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human HE4/WFDC2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human HE4/WFDC2.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human HE4/WFDC2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 470 1278 2448 476 1408 2731
Standard Deviation 11.5 35.3 86.1 26.0 79.1 162
CV% 2.4 2.8 3.5 5.5 5.6 5.9

Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 451 1337 2629 452 1409 2761
Standard Deviation 14.0 39.8 85.7 25.0 78.0 202
CV% 3.1 3.0 3.3 5.5 5.5 7.3

Recovery for Human HE4/WFDC2 Quantikine ELISA Kit

The recovery of HE4/WFDC2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 103 95-114
EDTA Plasma (n=4) 108 94-115
Heparin Plasma (n=4) 107 96-115
Saliva (n=4) 100 98-107
Serum (n=4) 104 92-115
Urine (n=4) 98 85-111

Linearity

To assess the linearity of the assay, samples containing high concentrations of HE4/WFDC2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human HE4/WFDC2 ELISA Linearity

Scientific Data Images for Human HE4/WFDC2 Quantikine ELISA Kit

Human HE4/WFDC2 ELISA Cell Culture Supernate/Serum/Plasma/Saliva Standard Curve

Human HE4/WFDC2 ELISA Cell Culture Supernate/Serum/Plasma/Saliva Standard Curve

Human HE4/WFDC2 ELISA Urine Standard Curve

Human HE4/WFDC2 ELISA Urine Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HE4/WFDC2

The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Entrez Gene IDs

10406 (Human); 67701 (Mouse); 286888 (Rat)

Gene Symbol

WFDC2

Additional HE4/WFDC2 Products

Product Documents for Human HE4/WFDC2 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HE4/WFDC2 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human HE4/WFDC2 Quantikine ELISA Kit

Customer Reviews for Human HE4/WFDC2 Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human HE4/WFDC2 Quantikine ELISA Kit and earn rewards!

Have you used Human HE4/WFDC2 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Human HE4/WFDC2 Quantikine ELISA Kit (DHE400):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of the appropriate Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits