Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HepaCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4108) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Hepatocyte Cell Adhesion Molecule (HepaCAM) is a 416 amino acid type I transmembrane glycoprotein that belongs to the immunoglobin superfamily. HepaCAM forms cis-homodimers on the cell surface and may be involved in modulating cell-matrix interactions, likely by promoting cell spreading and motility. Human HepaCAM runs as a 48-72 kDa protein depending on glycosylation. It contains a 206 amino acid extracellular domain and a 152 amino acid cytoplasmic domain. Over the extracellular region used as immunogen (aa 34-242), human HepaCAM is 99% and 98% identical to mouse and canine HepaCAM proteins, respectively.
Hepatocyte Cell Adhesion Molecule
Entrez Gene IDs:
cancer susceptibility; FLJ25530; glial cell adhesion molecule; GlialCAM; hepaCAM; hepatic and glial cell adhesion molecule; hepatocyte cell adhesion molecule; Protein hepaCAM
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.