Human HepaCAM Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human HepaCAM in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HepaCAM
    Val34-Tyr242
    Accession # Q14CZ8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human HepaCAM by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HepaCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4108) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HepaCAM
Hepatocyte Cell Adhesion Molecule (HepaCAM) is a 416 amino acid type I transmembrane glycoprotein that belongs to the immunoglobin superfamily. HepaCAM forms cis-homodimers on the cell surface and may be involved in modulating cell-matrix interactions, likely by promoting cell spreading and motility. Human HepaCAM runs as a 48-72 kDa protein depending on glycosylation. It contains a 206 amino acid extracellular domain and a 152 amino acid cytoplasmic domain. Over the extracellular region used as immunogen (aa 34-242), human HepaCAM is 99% and 98% identical to mouse and canine HepaCAM proteins, respectively.
  • Long Name:
    Hepatocyte Cell Adhesion Molecule
  • Entrez Gene IDs:
    220296 (Human)
  • Alternate Names:
    cancer susceptibility; FLJ25530; glial cell adhesion molecule; GlialCAM; HepaCAM; hepatic and glial cell adhesion molecule; hepatocyte cell adhesion molecule; Protein hepaCAM
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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