Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all HepaCAM Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HepaCAM
Val34-Tyr242
Accession # Q14CZ8

Specificity

Detects human HepaCAM in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human HepaCAM Antibody

Detection of Human HepaCAM antibody by Western Blot.

Detection of Human HepaCAM by Western Blot.

Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HepaCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4108) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human HepaCAM antibody by Simple WesternTM.

Detection of Human HepaCAM by Simple WesternTM.

Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for HepaCAM at approximately 63 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human HepaCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4108). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human HepaCAM Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human brain (motor cortex) tissue

Western Blot

1 µg/mL
Sample: Human brain (motor cortex) tissue and human brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HepaCAM

Hepatocyte Cell Adhesion Molecule (HepaCAM) is a 416 amino acid type I transmembrane glycoprotein that belongs to the immunoglobin superfamily. HepaCAM forms cis-homodimers on the cell surface and may be involved in modulating cell-matrix interactions, likely by promoting cell spreading and motility. Human HepaCAM runs as a 48-72 kDa protein depending on glycosylation. It contains a 206 amino acid extracellular domain and a 152 amino acid cytoplasmic domain. Over the extracellular region used as immunogen (aa 34-242), human HepaCAM is 99% and 98% identical to mouse and canine HepaCAM proteins, respectively.

Long Name

Hepatocyte Cell Adhesion Molecule

Alternate Names

cancer susceptibility, FLJ25530, glial cell adhesion molecule, GLIALCAM, hepaCAM, hepatic and glial cell adhesion molecule, hepatocyte cell adhesion molecule, Protein hepaCAM

Entrez Gene IDs

220296 (Human)

Gene Symbol

HEPACAM

UniProt

Q14CZ8

Additional HepaCAM Products

Product Documents for Human HepaCAM Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human HepaCAM Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

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