Human Hepcidin Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
  • Sensitivity
    3.81 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural human Hepcidin.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC220, Quantikine Immunoassay Control Set 881 for Human Hepcidin - Please Inquire
Product Summary
The Quantikine Human Hepcidin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Hepcidin in cell culture supernates, serum, plasma, and urine. It contains synthetic Hepcidin and antibodies raised against synthetic Hepcidin. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring human Hepcidin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean175350726164328647
Standard Deviation7.5110.823.51826.139.8
CV%4.33.13.21186.2

Recovery

The recovery of human Hepcidin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 111 99-118
EDTA Plasma (n=4) 103 95-115
Heparin Plasma (n=4) 104 93-117
Serum (n=4) 106 96-118
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Hepcidin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human Hepcidin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Hepcidin
    • Entrez Gene IDs
      57817 (Human);
    • Alternate Names
      HAMP; HEPC; hepcidin antimicrobial peptide; Hepcidin; HEPCPutative liver tumor regressor; HFE2B; HFE2Bhepcidin; LEAP1; LEAP-1; LEAP1PLTR; Liver-expressed antimicrobial peptide 1; PLTR;
    Related Research Areas
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 881 for Human Hepcidin

    Ctrl QC220
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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