Human Hepcidin Quantikine ELISA Kit

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Human Hepcidin ELISA Standard Curve
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Product Details
Citations (11)
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Reviews (1)

Human Hepcidin Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity
3.81 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural human Hepcidin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Hepcidin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Hepcidin in cell culture supernates, serum, plasma, and urine. It contains synthetic Hepcidin and antibodies raised against synthetic Hepcidin. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring human Hepcidin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 175 350 726 164 328 647
Standard Deviation 7.51 10.8 23.5 18 26.1 39.8
CV% 4.3 3.1 3.2 11 8 6.2

Recovery

The recovery of human Hepcidin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 111 99-118
EDTA Plasma (n=4) 103 95-115
Heparin Plasma (n=4) 104 93-117
Serum (n=4) 106 96-118

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Hepcidin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human Hepcidin ELISA Linearity

Scientific Data

Human Hepcidin ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Hepcidin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. The Role of Plasminogen Activator Inhibitor 1 in Predicting Sepsis-Associated Liver Dysfunction: An Observational Study
    Authors: E Wo?nica-Ni, P Le?nik, J Janc, M Zalewska, L ?ysenko
    International Journal of Environmental Research and Public Health, 2023-03-09;20(6):.
    Species: Human
    Sample Types: Plasma
  2. A randomized trial of once daily versus twice daily dosing of oral iron in CKD
    Authors: V Sood, K Kamboj, P Bhatia, V Sharma, M Kundu, A Ghosh, SK Singh, T Sen, P Kaur, R Ramachandr, M Rathi, HS Kohli, KL Gupta, S Malhotra, AK Yadav, V Kumar, V Jha
    Scientific Reports, 2023-01-04;13(1):141.
    Species: Human
    Sample Types: Serum
  3. Iron Metabolism following Twice a Day Endurance Exercise in Female Long-Distance Runners
    Authors: A Ishibashi, N Maeda, C Kojima, K Goto
    Nutrients, 2022-05-02;14(9):.
    Species: Human
    Sample Types: Serum
  4. Modulatory Effects of Fractalkine on Inflammatory Response and Iron Metabolism of Lipopolysaccharide and Lipoteichoic Acid-Activated THP-1 Macrophages
    Authors: E Pandur, K Tamási, R Pap, G Jánosa, K Sipos
    International Journal of Molecular Sciences, 2022-02-27;23(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Iron Deficiency in Inflammatory Bowel Disease Is Associated With Low Levels of Vitamin D Modulating Serum Hepcidin and Intestinal Ceruloplasmin Expression
    Authors: J Stallhofer, L Veith, J Diegelmann, P Probst, S Brand, F Schnitzler, T Olszak, H Török, J Mayerle, A Stallmach, F Beigel
    Clinical and Translational Gastroenterology, 2022-01-13;0(0):.
    Species: Human
    Sample Types: Serum
  6. Possible Association of Energy Availability with Transferrin Saturation and Serum Iron during Summer Camp in Male Collegiate Rugby Players
    Authors: M Tokuyama, J Seino, K Sakuraba, Y Suzuki
    Nutrients, 2021-08-26;13(9):.
    Species: Human
    Sample Types: Serum
  7. The immunoregulatory function of peripheral blood CD71+ erythroid cells in systemic-onset juvenile idiopathic arthritis
    Authors: H Kanemasa, M Ishimura, K Eguchi, T Tanaka, E Nanishi, A Shiraishi, M Goto, Y Motomura, S Ohga
    Scientific Reports, 2021-07-13;11(1):14396.
    Species: Human
    Sample Types: Serum
  8. Distinct Effects of Escherichia coli,Pseudomonas aeruginosa and Staphylococcus aureus Cell Wall Component-Induced Inflammation on the Iron Metabolism of THP-1 Cells
    Authors: E Pandur, K Tamási, R Pap, G Jánosa, K Sipos
    International Journal of Molecular Sciences, 2021-02-02;22(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. The Role of Iron and Erythropoietin in the Association of Fibroblast Growth Factor 23 with Anemia in Chronic Kidney Disease in Humans
    Authors: B Bielesz, T Reiter, FP Hammerle, W Winnicki, M Bojic, A Gleiss, H Kieweg, F Ratzinger, G Sunder-Pla, R Marculescu
    J Clin Med, 2020-08-14;9(8):.
    Species: Human
    Sample Types: Plasma
  10. Clinical utility of serum hepcidin and iron profile measurements in Alzheimer's disease
    Authors: OJ Kweon, YC Youn, YK Lim, MK Lee, HR Kim
    J. Neurol. Sci., 2019-06-10;403(0):85-91.
    Species: Human
    Sample Types: Serum
  11. A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis
    Authors: D Devos, C Moreau, M Kyheng, G Garçon, AS Rolland, H Blasco, P Gelé, T Timothée L, C Veyrat-Dur, P Corcia, M Dutheil, P Bede, A Jeromin, P Oeckl, M Otto, V Meninger, V Danel-Brun, JC Devedjian, JA Duce, PF Pradat
    Sci Rep, 2019-02-27;9(1):2918.
    Species: Human
    Sample Types: Plasma

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Human Hepcidin Quantikine ELISA Kit
By Anonymous on 11/22/2021
Sample Tested: human floxp embryonal stem cell culture medium