Human Hexokinase 1/2 Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # FAB8179AFP555

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 927312
View all Hexokinase 1/2 Primary Antibodies »

Product Specifications

Specificity

Detects human Hexokinase 2 in direct ELISAs. Detects human Hexokinase 1 and human Hexokinase 2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Hexokinase 1/2 Alexa Fluor™ Plus 555‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Hexokinase 1/2

Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step in hexose metabolism (1). Phosphorylation of a hexose adds charge to molecule thereby making it difficult to transport out of a cell. The hexose is therefore retained for intracellular metabolic processes, such as glycolysis or glycogen synthesis. In most organisms, glucose is the most important substrate of hexokinases and glucose-6-phosphate is the most important product. There are four mammalian hexokinases (2). Hexokinase 1, 2 and 3 are referred to as high-affinity hexokinases because their Km for glucose is below 1 mM. Hexokinase 4 is specific for glucose and is also referred to as glucokinase (3). Hexokinase 2 (HK2), also known as muscle form hexokinase, localizes to the outer membrane of mitochondria and is present in adipose tissue, skeletal muscle, and heart (4). The amino acids corresponding to the mitochondrial binding domain (5) have been removed in the recombinant enzyme. Like Hexokinase 1 (HK1), HK2 contains two homologous halves that may have evolved from an ancestral hexokinase through gene duplication and tandem ligation (6). Unlike HK1, in HK2 both the C-terminal and N-terminal portions are catalytically active with the N-terminal half having higher activity than the C-terminal half (7). In HK2 both the N-terminal and C-terminal halves exhibit product inhibition. HK2 overexpression is required for tumor growth making HK2 an attractive oncotarget (4). The enzymatic activity of recombinant human HK2 is measured using a phosphatase-coupled method (8).

References

  1. Aleshin, A.E. et al. (1998) Structure 6:39-50
  2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
  3. Lange, A.J. et al. (1991) Biochem. J. 277: 159.
  4. Patra, K.C. et al. (2013) Cancer Cell. 24:213.
  5. Bianchi M et al. (1998) Mol Cell Biochem 189:185.
  6. Ureta, T. (1982) Comp Biochem Physiol. B. 71B:549.
  7. Ahn, K.J. et al. (2009) BMB Rep. 42:350.
  8. Wu, Z.L. (2011) PLoS One 6:e23172.

UniProt

P52789

Additional Hexokinase 1/2 Products

Product Documents for Human Hexokinase 1/2 Alexa Fluor™ Plus 555‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Hexokinase 1/2 Alexa Fluor™ Plus 555‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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