Human Histamine H1 R PE-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Histamine H1 R in U937 human histiocytic lymphoma cell line activated with PMA by Flow Cytometry.
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Human Histamine H1 R PE-conjugated Antibody Summary

Species Reactivity
Detects human Histamine H1 R. Stains human Histamine H1 R transfectants but not irrelevant transfectants.
Monoclonal Mouse IgG1 Clone # 480054
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human Histamine H1 R
Accession # AAH60802
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Histamine H1 R antibody in U937 human histiocytic lymphoma cell line activated with PMA antibody by Flow Cytometry. View Larger

Detection of Histamine H1 R in U937 human histiocytic lymphoma cell line activated with PMA by Flow Cytometry. U937 human histiocytic lymphoma cell line, resting (A) or (B) activated with PMA, was stained with Mouse Anti-Human Histamine H1 R PE-conjugated Monoclonal Antibody (Catalog # FAB4726P, filled histograms) or isotype control antibody (Catalog # IC002P, open histograms). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Histamine H1 R

Histamine H1 R (HRH1) is a 60 kDa, 487 aa G-protein coupled 7-transmembrane putative glycoprotein. It is implicated in the pathogenesis of asthma and is a major pharmaceutical target for antihistamines. It has been detected as a mixture of monomers and homodimers, with highest concentration in the placenta, followed by brain, lung (produced by airway smooth muscle, bronchial epithelium and macrophages) and other tissues. Extracellular domains of human HRH1 share 72% aa sequence identity with corresponding regions of mouse or rat HRH1.

Long Name
Histamine H1 Receptor
Entrez Gene IDs
3269 (Human); 15465 (Mouse); 24448 (Rat)
Alternate Names
H1R; H1-R; hisH1; Histamine H1 R; Histamine H1R; histamine receptor H1; HRH1; subclass H1

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