Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 480054
View all Histamine H1 R Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Histamine H1 R
Met1-Ser487
Accession # AAH60802

Specificity

Detects human Histamine H1 R. Stains human Histamine H1 R transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Histamine H1 R PE‑conjugated Antibody

Detection of Histamine H1 R antibody in U937 human histiocytic lymphoma cell line activated with PMA antibody by Flow Cytometry.

Detection of Histamine H1 R in U937 human histiocytic lymphoma cell line activated with PMA by Flow Cytometry.

U937 human histiocytic lymphoma cell line, resting (A) or (B) activated with PMA, was stained with Mouse Anti-Human Histamine H1 R PE-conjugated Monoclonal Antibody (Catalog # FAB4726P, filled histograms) or isotype control antibody (Catalog # IC002P, open histograms). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Histamine H1 R PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: U937 human histiocytic lymphoma cell line activated with PMA

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Histamine H1 R

Histamine H1 R (HRH1) is a 60 kDa, 487 aa G-protein coupled 7-transmembrane putative glycoprotein. It is implicated in the pathogenesis of asthma and is a major pharmaceutical target for antihistamines. It has been detected as a mixture of monomers and homodimers, with highest concentration in the placenta, followed by brain, lung (produced by airway smooth muscle, bronchial epithelium and macrophages) and other tissues. Extracellular domains of human HRH1 share 72% aa sequence identity with corresponding regions of mouse or rat HRH1.

Long Name

Histamine H1 Receptor

Alternate Names

H1-R, Histamine H1R, HRH1

Entrez Gene IDs

3269 (Human); 15465 (Mouse); 24448 (Rat)

Gene Symbol

HRH1

UniProt

AAH60802

Additional Histamine H1 R Products

Product Documents for Human Histamine H1 R PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Histamine H1 R PE‑conjugated Antibody

For research use only

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