Human Histamine H1 R PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Histamine H1 R. Stains human Histamine H1 R transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG1 Clone # 480054
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Histamine H1 R
    Accession # AAH60802
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Histamine H1 R in U937 human histiocytic lymphoma cell line activated with PMA by Flow Cytometry. U937 human histiocytic lymphoma cell line, resting (A) or (B) activated with PMA, was stained with Mouse Anti-Human Histamine H1 R PE‑conjugated Monoclonal Antibody (Catalog # FAB4726P, filled histograms) or isotype control antibody (Catalog # IC002P, open histograms). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Histamine H1 R

Histamine H1 R (HRH1) is a 60 kDa, 487 aa G-protein coupled 7-transmembrane putative glycoprotein. It is implicated in the pathogenesis of asthma and is a major pharmaceutical target for antihistamines. It has been detected as a mixture of monomers and homodimers, with highest concentration in the placenta, followed by brain, lung (produced by airway smooth muscle, bronchial epithelium and macrophages) and other tissues. Extracellular domains of human HRH1 share 72% aa sequence identity with corresponding regions of mouse or rat HRH1.

  • Long Name:
    Histamine H1 Receptor
  • Entrez Gene IDs:
    3269 (Human); 15465 (Mouse); 24448 (Rat)
  • Alternate Names:
    H1R; H1-R; hisH1; Histamine H1R; histamine receptor H1; HRH1; subclass H1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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