Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 480054
View all Histamine H1 R Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Histamine H1 R
Met1-Ser487
Accession # AAH60802

Specificity

Detects human Histamine H1 R. Stains human Histamine H1 R transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Histamine H1 R Antibody

Detection of Histamine H1 R antibody in PMA-treated U937 Human Cell Line antibody by Flow Cytometry.

Detection of Histamine H1 R in PMA‑treated U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line treated with 50 ng/mL PMA for 24 hours was stained with Mouse Anti-Human Histamine H1 R Monoclonal Antibody (Catalog # MAB4726, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
Detection of Histamine H1 R antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of Histamine H1 R in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Histamine H1 R Monoclonal Antibody (Catalog # MAB4726, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human Histamine H1 R Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line treated with PMA

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histamine H1 R

Histamine H1 R (HRH1) is a 60 kDa, 487 aa G-protein coupled 7-transmembrane putative glycoprotein. It is implicated in the pathogenesis of asthma and is a major pharmaceutical target for antihistamines. It has been detected as a mixture of monomers and homodimers, with highest concentration in the placenta, followed by brain, lung (produced by airway smooth muscle, bronchial epithelium and macrophages) and other tissues. Extracellular domains of human HRH1 share 72% aa sequence identity with corresponding regions of mouse or rat HRH1.

Long Name

Histamine H1 Receptor

Alternate Names

H1-R, Histamine H1R, HRH1

Entrez Gene IDs

3269 (Human); 15465 (Mouse); 24448 (Rat)

Gene Symbol

HRH1

UniProt

AAH60802

Additional Histamine H1 R Products

Product Documents for Human Histamine H1 R Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Histamine H1 R Antibody

For research use only

Citations for Human Histamine H1 R Antibody

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