Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2400G

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HNF-3 beta /Fox A2 in Mesendoderm Differentiated BG01V Human Stem Cells.
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Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HNF‑3 beta /FoxA2
Met242-Ser457
Accession # Q9Y261
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
10 µg/mL
See below
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry HNF-3 beta /Fox A2 antibody in Mesendoderm Differentiated BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

HNF-3 beta /Fox A2 in Mesendoderm Differentiated BG01V Human Stem Cells. BG01V human embryonic stem cells were differentiated into mesendoderm using the StemXVivo Endoderm Kit (Catalog # SC019). HNF-3 beta /FoxA2 was detected in immersion fixed mesendoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, green) for 3 hours at room temperature and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of HNF-3 beta /FoxA2 antibody in Human iPS Cells antibody by Flow Cytometry. View Larger

Detection of HNF‑3 beta /FoxA2 in Human iPS Cells by Flow Cytometry. Human iPS cells were stained with Goat Anti-Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry View Larger

Detection of HNF‑3 beta /FoxA2 in HepG2 Human Cell Line by Flow Cytometry HepG2 human liver cell line was stained with Goat Anti-Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HNF-3 beta/FoxA2

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Long Name
Hepatocyte Nuclear Factor-3, beta
Entrez Gene IDs
3170 (Human)
Alternate Names
forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF3 beta; HNF-3 beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar
    Cell Stem Cell, 2021-02-04;28(2):217-229.e7.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  2. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm
    Authors: Mahaddalkar PU, Scheibner K, Pfluger S et al.
    Nature Biotechnology
  3. Derivation of notochordal cells from human embryonic stem cells reveals unique regulatory networks by single cell‐transcriptomics
    Authors: Martha E. Diaz‐Hernandez, Nazir M. Khan, Camila M. Trochez, Tim Yoon, Peter Maye, Steven M. Presciutti et al.
    Journal of Cellular Physiology
  4. FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation
    Authors: K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu
    Cell Rep, 2019-07-09;28(2):382-393.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis
    Authors: Yijie Geng, Bradley Feng
    Heliyon

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