HNF-3 beta /Fox A2 in Mesendoderm Differentiated BG01V Human Stem Cells.
BG01V human embryonic stem cells were differentiated into mesendoderm using the StemXVivo Endoderm Kit (Catalog # SC019). HNF-3 beta /FoxA2 was detected in immersion fixed mesendoderm differentiated BG01V human embryonic stem cells using 10 μg/mL Goat Anti-Human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, green) for 3 hours at room temperature and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of HNF‑3 beta /FoxA2 in Human iPS Cells by Flow Cytometry.
Human iPS cells were stained with Goat Anti-Human HNF‑3 beta /FoxA2 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: HNF-3 beta/FoxA2
HNF-3 beta, also known as FoxA2, is a member of the forkhead class of
DNA-binding proteins. It is a transcriptional activator for
liver-specific transcripts such as albumin and transthyretin. Similar
family members play roles in the differentiation of the pancreas and
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