Human HNF-3 beta /FoxA2 Antibody

Catalog # Availability Size / Price Qty
AF2400
AF2400-SP

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Detection of HNF‑3 beta /FoxA2-regulated Genes by Chromatin Immunoprecipitation.
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Human HNF-3 beta /FoxA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HNF‑3 beta /FoxA2
Met242-Ser457
Accession # Q9Y261
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
HepG2 human hepatocellular carcinoma cell line
Immunohistochemistry
3-15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed endoderm differentiated BG01V human embryonic stem cells and immersion fixed HepG2 Human Hepatocellular Carcinoma Cells (Positive) and absent in THP‑1 Human Acute Monocytic Leukemia Cells (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Chromatin Immunoprecipitation (ChIP) Detection of HNF-3 beta /FoxA2-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of HNF‑3 beta /FoxA2-regulated Genes by Chromatin Immunoprecipitation. Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF-3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. TheE-RABPpromoter was detected by standard PCR.

Immunocytochemistry HNF-3 beta /FoxA2 antibody in Endoderm Differentiated BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

HNF‑3 beta /FoxA2 in Endoderm Differentiated BG01V Human Stem Cells. HNF-3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry HNF-3 beta /FoxA2 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

HNF‑3 beta /FoxA2 in Human Liver. HNF-3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry View Larger

Detection of HNF‑3 beta /FoxA2 in HepG2 Cells (Positive) and THP‑1 Cells (Negative). HNF‑3 beta /FoxA2 was detected in immersion fixed HepG2 Human Hepatocellular Carcinoma Cells (Positive) and absent in THP‑1 Human Acute Monocytic Leukemia Cells (Negative) using Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot View Larger

Detection of Human HNF‑3 beta /FoxA2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry/ Immunofluorescence Detection of Human HNF-3 beta/FoxA2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human HNF-3 beta/FoxA2 by Immunocytochemistry/Immunofluorescence Differentiation of hESCs into definitive endoderm cells by Activin A with CHIR99021.a The relative primitive streak (BRACHYRUY, MESP1, and MIXL1) gene expression of the day 1 differentiated cells by adding Activin A (100 ng/mL) with CHIR99021 (2–5 µM) or Wnt3a (25–100 ng/mL) treatments were determined by real-time quantitative PCR (qPCR). None, no WNT signaling pathway activators were used on day 1 for PS differentiation (Activin A only). b, c After 1 day, the medium was changed to Activin A (100 ng/mL) and 1 × CTS-B27 to induce DE differentiation. The definitive endoderm (FOXA2 and SOX17) and mesoderm (MESP2 and HAND1) relative gene expression levels were determined by qPCR. d Immunofluorescence analysis of the expression of SOX17 and FoxA2 for Activin A with 2 µM CHIR99021-induced differentiated cells on day 3. e, f The expression of SOX17 and FoxA2 for Activin A with 2 µM CHIR99021-induced differentiated cells was determined by flow cytometry on day 3. Isotype control antibodies were used as controls. At a specific gene expression, datum points lacking common letters differ, p < 0.05. Data are represented as the mean ± SD. Scale bar, 100 µm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31601782), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HNF-3 beta/FoxA2

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Long Name
Hepatocyte Nuclear Factor-3, beta
Entrez Gene IDs
3170 (Human)
Alternate Names
forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF3 beta; HNF-3 beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B

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Citations for Human HNF-3 beta /FoxA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

90 Citations: Showing 1 - 10
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  1. One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
    Authors: Lin Feng, Da Li, Yao Tian, Chengshun Zhao, Yun Sun, Xiaolong Kou et al.
    Neural Regeneration Research
  2. In depth characterization of midbrain organoids derived from wild type iPSC lines
    Authors: Pavlinov, I;Tambe, M;Abbott, J;Nguyen, HN;Xu, M;Pradhan, M;Farkhondeh, A;Zheng, W;
    PloS one
    Species: Human
    Sample Types: Organoids
  3. Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease
    Authors: Boeing, A;Mavrommatis, L;Daya, NM;Zhuge, H;Volke, L;Kocabas, A;Kneifel, M;Athamneh, M;Krause, K;Südkamp, N;Döring, K;Theiss, C;Roos, A;Zaehres, H;Güttsches, AK;Vorgerd, M;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative
    Authors: Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
    Authors: Souralova, T;Hulinova, D;Jeseta, M;Ventruba, P;Hampl, A;Koutna, I;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
    Authors: Kaneyasu Nishimura, Emilía Sif Ásgrímsdóttir, Shanzheng Yang, Ernest Arenas
    STAR Protocols
  7. Protocol for generating reproducible miniaturized controlled midbrain organoids
    Authors: Muwan Chen, Jonathan Christos Niclis, Mark Denham
    STAR Protocols
  8. Generation of an induced pluripotent stem cell line (ESi107-A) from a transthyretin amyloid cardiomyopathy (ATTR-CM) patient carrying a p.Ser43Asn mutation in the TTR gene
    Authors: Montero-Calle, P;Flandes-Iparraguirre, M;Kuebler, B;Arán, B;Larequi, E;Anaut, I;Coppiello, G;Aranguren, XL;Veiga, A;Elorz, MTB;de Yébenes, MG;Gavira, JJ;Prósper, F;Iglesias-García, O;Vega, MMM;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells
    Authors: Takeshi Kato, Kaneyasu Nishimura, Masahiro Hirao, Shun Shimohama, Kazuyuki Takata
    Neuropsychopharmacology Reports
  10. Post-translational proteomics platform identifies neurite outgrowth impairments in Parkinson's disease GBA-N370S dopamine neurons
    Authors: H Bogetofte, BJ Ryan, P Jensen, SI Schmidt, DLE Vergoossen, MB Barnkob, LN Kiani, U Chughtai, R Heon-Rober, MC Caiazza, W McGuinness, R Márquez-Gó, J Vowles, FS Bunn, J Brandes, P Kilfeather, JP Connor, HJR Fernandes, TM Caffrey, M Meyer, SA Cowley, MR Larsen, R Wade-Marti
    Cell Reports, 2023-03-03;42(3):112180.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome
    Authors: EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova
    Stem Cell Research, 2022-12-30;67(0):103012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  12. Generation of human elongating multi-lineage organized cardiac gastruloids
    Authors: Zachary T. Olmsted, Maria Belen Paredes-Espinosa, Janet L. Paluh
    STAR Protocols
  13. Generation of an induced pluripotent stem cell line (IPCASi001-A) from an autism spectrum disorder individual without intellectual disability
    Authors: L Yang, S Lu, Z Yang, J Yao, P Zhou, M Zhao
    Stem Cell Research, 2022-12-05;66(0):102994.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. A reference human induced pluripotent stem cell line for large-scale collaborative studies
    Authors: CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle
    Cell Stem Cell, 2022-12-01;29(12):1685-1702.e22.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  15. Analysis of Influenza A virus infection in human induced pluripotent stem cells (hiPSCs) and their derivatives
    Authors: K Ruangrung, W Chakritbud, S Thongon, S Rungarunle, M Wattanapan, C Boonarkart, O Suptawiwat, N Sirinontha, DR Smith, P Auewarakul
    Virus research, 2022-11-19;323(0):199009.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Induced Pluripotent Stem Cell (iPSC) Lines from a Family with Resistant Epileptic Encephalopathy Caused by Compound Heterozygous Mutations in SZT2 Gene
    Authors: C Cattelani, I Battistell, F Di Leva, G Fioravanti, F Benedicent, F Stanzial, C Schwienbac, F Fanelli, PP Pramstalle, AA Hicks, L Conti, C Corti
    International Journal of Molecular Sciences, 2022-10-28;23(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing
    Authors: Hunter J. Johnson, Saheli Chakraborty, Riya J. Muckom, Nitash P. Balsara, David V. Schaffer
    iScience
  18. The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy
    Authors: Tereza Souralova, Daniela Rehakova, Michal Jeseta, Lenka Tesarova, Jindrich Beranek, Pavel Ventruba et al.
    International Journal of Molecular Sciences
  19. A combined human gastruloid model of cardiogenesis and neurogenesis
    Authors: Olmsted ZT, Paluh JL.
    iScience
  20. Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats
    Authors: T Tada, H Ohnishi, N Yamamoto, F Kuwata, Y Hayashi, H Okuyama, T Morino, Y Kasai, H Kojima, K Omori
    Regenerative Therapy, 2022-01-14;19(0):77-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants
    Authors: P Aldrin-Kir, M Åkerblom, T Cardoso, S Nolbrant, AF Adler, X Liu, A Heuer, M Davidsson, M Parmar, T Björklund
    Stem Cell Reports, 2021-12-30;17(1):159-172.
    Species: Human, Rat, Transgenic Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  22. Pharmacological targeting of Sam68 functions in colorectal cancer stem cells
    Authors: Angelique N. Masibag, Christopher J. Bergin, Joshua R. Haebe, Aïcha Zouggar, Muhammad S. Shah, Tamara Sandouka et al.
    iScience
  23. Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells
    Authors: Miranda Robbins, Venkat Pisupati, Roberta Azzarelli, Samer I. Nehme, Roger A. Barker, Ljiljana Fruk et al.
    Stem Cell Research & Therapy
  24. Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors
    Authors: RJ Geusz, A Wang, DK Lam, NK Vinckier, KD Alysandrat, DA Roberts, J Wang, S Kefalopoul, A Ramirez, Y Qiu, J Chiou, KJ Gaulton, B Ren, DN Kotton, M Sander
    Nature Communications, 2021-11-17;12(1):6636.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ChIP, IHC
  25. Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96?Transwell air-liquid interface model
    Authors: T Bluhmki, S Traub, AK Müller, S Bitzer, E Schruf, MT Bammert, M Leist, F Gantner, JP Garnett, R Heilker
    Scientific Reports, 2021-08-23;11(1):17028.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  26. Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels
    Authors: A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A
    Cell Death & Disease, 2021-06-21;12(7):637.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  27. SARS-CoV-2 Infection Causes Dopaminergic Neuron Senescence
    Authors: S Chen, Y Han, L Yang, T Kim, M Nair, O Harschnitz, P Wang, J Zhu, SY Koo, X Tang, L Lacko, V Chandar, Y Bram, T Zhang, W Zhang, F He, J Caicedo, Y Huang, T Evans, P van der Va, MJ Titulaer, JKH Spoor, RL Furler, P Canoll, J Goldman, S Przedborsk, R Schwartz, D Ho, L Studer
    Research square, 2021-05-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  28. Airway basal stem cells generate distinct subpopulations of PNECs
    Authors: H Mou, Y Yang, MA Riehs, J Barrios, M Shivaraju, AL Haber, DT Montoro, K Gilmore, EA Haas, B Paunovic, J Rajagopal, SO Vargas, RL Haynes, A Fine, WV Cardoso, X Ai
    Cell Reports, 2021-04-20;35(3):109011.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  29. Human naive epiblast cells possess unrestricted lineage potential
    Authors: G Guo, GG Stirparo, SE Strawbridg, D Spindlow, J Yang, J Clarke, A Dattani, A Yanagida, MA Li, S Myers, BN Özel, J Nichols, A Smith
    Cell Stem Cell, 2021-04-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys
    Authors: Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang
    Nature Medicine, 2021-03-01;0(0):.
    Species: Primate - Rhesus macaque
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  31. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use
    Authors: TW Kim, J Piao, SY Koo, S Kriks, SY Chung, D Betel, ND Socci, SJ Choi, S Zabierowsk, BN Dubose, EJ Hill, EV Mosharov, S Irion, MJ Tomishima, V Tabar, L Studer
    Cell Stem Cell, 2021-02-04;28(2):343-355.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar
    Cell Stem Cell, 2021-02-04;28(2):217-229.e7.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  33. Derivation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering 7q11.23 microduplication syndrome
    Authors: B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, I Cuscó, R Corominas
    Stem Cell Res, 2020-11-19;49(0):102092.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Generation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering Williams-Beuren syndrome (7q11.23 deletion)
    Authors: B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, R Corominas, I Cuscó
    Stem Cell Research, 2020-11-16;49(0):102087.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Generation, establishment and characterization of a pluripotent stem cell line (CVTTHi001-A) from primary fibroblasts isolated from a patient with activated PI3 kinase delta syndrome (APDS2)
    Authors: M Inglés-Fer, M Martin-Ina, L Herrera, M Villaverde, S Santos, MA Vesga, E Garreta, I Martín-Rui, AM Aransay, J Anguita, B Barreña, LM Allende, LI Gonzalez-G, C Eguizabal
    Stem Cell Res, 2020-11-07;49(0):102082.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population
    Authors: Patrick Walsh, Vincent Truong, Sushmita Nayak, Marietta Saldías Montivero, Walter C. Low, Ann M. Parr et al.
    Stem Cells
  37. Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells
    Authors: E Naumovska, G Aalderink, C Wong Valen, K Kosim, A Nicolas, S Brown, P Vulto, KS Erdmann, D Kurek
    Int J Mol Sci, 2020-07-14;21(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. HDAC3-dependent transcriptional repression of FOXA2 regulates FTO/m6A/MYC signaling to contribute to the development of gastric cancer
    Authors: Z Yang, X Jiang, Z Zhang, Z Zhao, W Xing, Y Liu, X Jiang, H Zhao
    Cancer Gene Ther., 2020-07-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  39. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020-06-19;27(1):125-136.e7.
    Species: Human
    Sample Types: Organoids, Whole Cells
    Applications: ICC, IHC
  40. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
    Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
    Sci Rep, 2020-04-06;10(1):5989.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model
    Authors: YY Kim, H Kim, CS Suh, HC Liu, Z Rosenwaks, SY Ku
    Int J Mol Sci, 2020-01-24;21(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Hepatic TET3 contributes to type-2 diabetes by inducing the HNF4alpha fetal isoform
    Authors: Da Li, T Cao, X Sun, S Jin, Di Xie, X Huang, X Yang, GG Carmichael, HS Taylor, S Diano, Y Huang
    Nat Commun, 2020-01-17;11(1):342.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  43. Generation of three induced pluripotent cell lines (iPSCs) from an Aicardi-Gouti�res syndrome (AGS) patient harboring a deletion in the genomic locus of the sterile alpha motif and HD domain containing protein 1 (SAMHD1)
    Authors: NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, L Wendeburg, D Steinemann, C Elpers, F Rutsch, R König
    Stem Cell Res, 2020-01-09;43(0):101697.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020;27(1):125-136.e7.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  45. Hepatic TET3 contributes to type-2 diabetes by inducing the HNF4alpha fetal isoform
    Authors: Da Li, T Cao, X Sun, S Jin, Di Xie, X Huang, X Yang, GG Carmichael, HS Taylor, S Diano, Y Huang
    Nat Commun, 2020;11(1):342.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  46. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency
    Authors: N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo
    Stem Cell Reports, 2019-11-07;13(6):1083-1098.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons
    Authors: M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard
    Cell Stem Cell, 2019-09-19;25(4):514-530.e8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency
    Authors: Hannah T. Stuart, Giuliano G. Stirparo, Tim Lohoff, Lawrence E. Bates, Masaki Kinoshita, Chee Y. Lim et al.
    Cell Stem Cell
  49. FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation
    Authors: K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu
    Cell Rep, 2019-07-09;28(2):382-393.e7.
    Species: Human
    Sample Types: Cell Lysates, Chromatin
    Applications: Immunoprecipitation, Western Blot
  50. An integrated transcriptomics and proteomics analysis reveals functional endocytic dysregulation caused by mutations in LRRK2
    Authors: Natalie Connor-Robson, Heather Booth, Jeffrey G. Martin, Benbo Gao, Kejie Li, Natalie Doig et al.
    Neurobiology of Disease
  51. Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons
    Authors: R Yokoi, M Okabe, N Matsuda, A Odawara, A Karashima, I Suzuki
    Front Neurosci, 2019-05-29;13(0):554.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  52. CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons
    Authors: C Calatayud, G Carola, I Fernández-, M Valtorta, S Jiménez-De, M Díaz, J Soriano-Fr, G Cappellett, J García-San, Á Raya, A Consiglio
    Sci Rep, 2019-05-02;9(1):6811.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival
    Authors: T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen
    Nat Commun, 2018-11-16;9(1):4815.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors
    Authors: H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko
    Stem Cell Res Ther, 2018-10-25;9(1):277.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  55. AAVvector-mediated in vivo reprogramming into pluripotency
    Authors: E Senís, L Mosteiro, S Wilkening, E Wiedtke, A Nowrouzi, S Afzal, R Fronza, H Landerer, M Abad, D Niopek, M Schmidt, M Serrano, D Grimm
    Nat Commun, 2018-07-09;9(1):2651.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  56. Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS)
    Authors: B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya
    Stem Cell Res, 2017-09-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  57. Idiopathic Parkinson's disease patient‐derived induced pluripotent stem cells function as midbrain dopaminergic neurons in rodent brains
    Authors: Tetsuhiro Kikuchi, Asuka Morizane, Daisuke Doi, Keisuke Okita, Masato Nakagawa, Hodaka Yamakado et al.
    Journal of Neuroscience Research
  58. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
    Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
    Stem Cell Reports, 2017-06-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  59. Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines
    Authors: L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya
    Stem Cell Res, 2017-06-07;24(0):155-159.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  60. Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
    Authors: Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune
    Diabetologia, 2017-05-22;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC
  61. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017-04-04;19(1):20-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  62. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017-03-23;12(4):814-827.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  63. Live Imaging Mitochondrial Transport in Neurons
    Authors: Meredith M. Course, Chung-Han Hsieh, Pei-I Tsai, Jennifer A. Codding-Bui, Atossa Shaltouki, Xinnan Wang
    Neuromethods
  64. Generation of thalamic neurons from mouse embryonic stem cells
    Authors: A Shiraishi, K Muguruma, Y Sasai
    Development, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  65. Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver
    Authors: Leslie E Rogler
    J. Histochem. Cytochem., 2016-11-24;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  66. Cell Expansion During Directed Differentiation Of Stem Cells Toward The Hepatic Lineage
    Stem Cells Dev., 2016-11-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  67. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016-08-10;6(0):31063.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  68. Aberrant epigenome in iPSC ‐derived dopaminergic neurons from Parkinson's disease patients
    Authors: Rubén Fernández‐Santiago, Iria Carballo‐Carbajal, Giancarlo Castellano, Roger Torrent, Yvonne Richaud, Adriana Sánchez‐Danés et al.
    EMBO Molecular Medicine
  69. A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells.
    Authors: Tsankov A, Akopian V, Pop R, Chetty S, Gifford C, Daheron L, Tsankova N, Meissner A
    Nat Biotechnol, 2015-10-26;33(11):1182-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  70. The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing
    Authors: Filipe V. Jacinto, Chris Benner, Martin W. Hetzer
    Genes & Development
  71. Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
    Authors: Villa-Diaz L, Kim J, Lahann J, Krebsbach P
    Stem Cells Transl Med, 2014-10-13;3(12):1410-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  72. Physiological characterisation of human iPS-derived dopaminergic neurons.
    Authors: Hartfield, Elizabet, Yamasaki-Mann, Michiko, Ribeiro Fernandes, Hugo J, Vowles, Jane, James, William, Cowley, Sally A, Wade-Martins, Richard
    PLoS ONE, 2014-02-21;9(2):e87388.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  73. Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment
    Authors: Lynsey Howard, Ruth M Mackenzie, Nikolay A Pchelintsev, Tony McBryan, John D McClure, Martin W McBride et al.
    Stem Cell Research & Therapy
  74. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2.
    Authors: Montserrat N, Ramirez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, Gonzalez F, Izpisua Belmonte JC, Campistol JM
    J. Biol. Chem., 2012-05-21;287(29):24131-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  75. Treatment with small molecules is an important milestone towards the induction of pluripotency in neural stem cells derived from human cord blood.
    Authors: Szablowska-Gadomska, Ilona, Sypecka, Joanna, Zayat, Valery, Podobinska, Martyna, Pastwinska, Anna, Pienkowska-Grela, Barbara, Buzanska, Leonora
    Acta Neurobiol Exp (Wars), 2012-01-01;72(4):337-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  76. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2011-12-21;21(6):1321-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  77. Complete meiosis from human induced pluripotent stem cells.
    Authors: Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC
    Stem Cells, 2011-08-01;29(8):1186-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  78. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
    Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
    J. Cell. Sci., 2011-05-24;124(0):1992-2000.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  79. Recapitulation of premature ageing with iPSCs from Hutchinson–Gilford progeria syndrome
    Authors: Guang-Hui Liu, Basam Z. Barkho, Sergio Ruiz, Dinh Diep, Jing Qu, Sheng-Lian Yang et al.
    Nature
  80. CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
    Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
    Stem Cells, 2011-04-01;29(4):609-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  81. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010-11-14;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  82. Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
    Authors: Chayosumrit M, Tuch B, Sidhu K
    Biomaterials, 2009-10-14;31(3):505-14.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  83. Linking the p53 tumour suppressor pathway to somatic cell reprogramming.
    Authors: Kawamura T, Suzuki J, Wang YV, Menendez S, Morera LB, Raya A, Wahl GM, Belmonte JC
    Nature, 2009-08-09;460(7259):1140-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  84. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.
    Authors: Raya A, Rodriguez-Piza I, Guenechea G, Vassena R, Navarro S, Barrero MJ, Consiglio A, Castella M, Río P, Sleep E, Gonzalez F, Tiscornia G, Garreta E, Aasen T, Veiga A, Verma IM, Surralles J, Bueren J, Belmonte JC
    Nature, 2009-05-31;460(7251):53-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  85. Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.
    Authors: Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H
    Cell Res., 2009-04-01;19(4):429-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  86. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008-10-08;456(7220):344-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  87. Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium.
    Authors: Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M
    FASEB J., 2008-09-22;23(1):114-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  88. Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.
    Authors: Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H
    Blood, 2007-11-27;111(4):1933-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  89. Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure.
    Authors: Cho CH, Parashurama N, Park EY, Suganuma K, Nahmias Y, Park J, Tilles AW, Berthiaume F, Yarmush ML
    FASEB J., 2007-10-17;22(3):898-909.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  90. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
    Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
    Proc. Natl. Acad. Sci. U.S.A., 2006-04-21;103(18):6907-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human HNF-3 beta /FoxA2 Antibody
By Anonymous on 09/10/2021
Application: IHC Sample Tested: Prostate cancer tissue Species: Human

Human HNF-3 beta /FoxA2 Antibody
By Anonymous on 02/15/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: alveoli Species: Human

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