hh Human ICAM-1/CD54 Fluorescein-conjugated Antibody BBA20: R&D Systems

Human ICAM-1/CD54 Fluorescein-conjugated Antibody

(15 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Stains human ICAM-1/CD54 on human ICAM-1 transfected COS cells. It does not bind COS cells transfected with E-Selectin, VCAM-1, or PECAM-1.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ICAM‑1/CD54 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human ICAM‑1/CD54 Fluorescein‑conjugated Monoclonal Antibody (Catalog # BBA20) or (B) Mouse IgG1 Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ICAM-1/CD54
ICAM-1 is a member of the immunoglobulin superfamily whose expression is up‑regulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.
  • Long Name:
    Intercellular Adhesion Molecule 1
  • Entrez Gene IDs:
    3383 (Human); 15894 (Mouse); 25464 (Rat)
  • Alternate Names:
    BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease
    Authors: SH Chan, CH Hung, JY Shih, PM Chu, YH Cheng, HC Lin, KL Tsai
    Redox Biol, 2017;13(0):301-309.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro
    Authors: A Schaefer, TJ van Duijn, J Majolee, K Burridge, PL Hordijk
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells
    PLoS ONE, 2016;11(12):e0167361.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016;291(20):10586-601.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Molecular mechanisms regulating the synergism between IL-32gamma and NOD for the activation of eosinophils.
    Authors: Wong C, Dong J, Lam C
    J Leukoc Biol, 2014;95(4):631-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.
    Authors: Stephan D, Sbai O, Wen J, Couraud P, Putterman C, Khrestchatisky M, Desplat-Jego S
    J Neuroinflammation, 2013;10(0):9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
    Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
    J Allergy Clin Immunol, 2013;131(2):562-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.
    Authors: Liu K, Hu S, Chan B, Wat E, Lau C, Hon K, Fung K, Leung P, Hui P, Lam C, Wong C
    Molecules, 2013;18(3):2483-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Transendothelial migration enables subsequent transmigration of neutrophils through underlying pericytes.
    Authors: Ayres-Sander C, Lauridsen H, Maier C, Sava P, Pober J, Gonzalez A
    PLoS ONE, 2013;8(3):e60025.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
    Authors: Li N, Mao D, Lu S, Tong C, Zhang Y, Long M
    J Immunol, 2013;190(8):4371-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012;287(32):26478-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
    Authors: Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
    PLoS ONE, 2010;5(10):e13568.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
    Authors: Marino M, Scuderi F, Provenzano C, Scheller J, Rose-John S, Bartoccioni E
    J. Neuroimmunol., 2008;196(1):41-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
    Authors: Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R
    Atherosclerosis, 2007;190(1):90-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
    Authors: Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
    J. Leukoc. Biol., 2003;73(4):525-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 15 Citations
Isotype Controls
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Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 14  
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

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