Human IFN-alpha 2 Quantikine HS ELISA Kit

Catalog # Availability Size / Price Qty
HSIFNA2
Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity
3 Images
Product Details
FAQs
Reviews

Human IFN-alpha 2 Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.107 pg/mL
Assay Range
0.8 - 50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IFN-alpha 2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to asses inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 2.38 15.1 32.3 2.65 15.5 29.7
Standard Deviation 0.082 0.686 1.36 0.15 0.965 1.69
CV% 3.4 4.5 4.2 5.7 6.2 5.7

Recovery

The recovery of human IFN-alpha 2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 90-108
EDTA Plasma (n=4) 89 75-101
Heparin Plasma (n=4) 86 75-92
Serum (n=4) 86 75-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-alpha 2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Data Examples

Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity IFN-Alpha 2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-112% for Heparin Plasma, 97%-114% for EDTA Plasma, and 94%-111% for Serum.

Human IFN-alpha 2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha 2/IFNA2

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name:
Interferon alpha 2
Entrez Gene IDs:
3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names:
alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Human IFN-alpha 2 Quantikine HS ELISA Kit

There are currently no reviews for this product. Be the first to review Human IFN-alpha 2 Quantikine HS ELISA Kit and earn rewards!

Have you used Human IFN-alpha 2 Quantikine HS ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review