Human IFN-alpha 2 Quantikine HS ELISA Kit Summary
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human IFN-alpha 2 spiked to levels throughout the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||101||90-108|
|EDTA Plasma (n=4)||89||75-101|
|Heparin Plasma (n=4)||86||75-92|
Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity IFN-Alpha 2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-112% for Heparin Plasma, 97%-114% for EDTA Plasma, and 94%-111% for Serum.
Human IFN-alpha 2 ELISA Standard Curve
Preparation and Storage
Background: IFN-alpha 2/IFNA2
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
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