Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFN‑ gamma R1, rhIFN-gamma R2, recombinant mouse IFN-alpha / beta R2, rhIL-10 R beta, rhIL-10 R alpha, or rhIL-20 R beta is observed.
Monoclonal Mouse IgG1 Clone # 85228
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1 Lys28-Lys436 Accession # AAA52730
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IFN‑ alpha / beta R1 in Human U937 cell line by Flow Cytometry. Human U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human IFN‑ alpha / beta R1 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB245C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IFN-alpha/beta R1
Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.
Interferon alpha/beta Receptor 1
Entrez Gene IDs:
3454 (Human); 15975 (Mouse)
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1
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