Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 85228
View all IFN-alpha/beta R1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta  R1
Lys28-Lys436
Accession # AAA52730

Specificity

Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) IFN‑ gamma  R1, rhIFN-gamma R2, recombinant mouse IFN-alpha / beta  R2, rhIL-10 R beta, rhIL-10 R alpha, or rhIL-20 R beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human IFN‑ alpha / beta  R1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human IFN-alpha / beta  R1

Reviewed Applications

Read 4 reviews rated 4 using MAB245 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Gene Symbol

IFNAR1

UniProt

AAA52730

Additional IFN-alpha/beta R1 Products

Product Documents for Human IFN‑ alpha / beta  R1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IFN‑ alpha / beta  R1 Antibody

For research use only

Citations for Human IFN‑ alpha / beta  R1 Antibody

Customer Reviews for Human IFN‑ alpha / beta  R1 Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human IFN-alpha / beta  R1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hepatoma cells
    Species: Human
    Verified Customer | Posted 10/04/2021
    Human IFN‑ alpha / beta  R1 Antibody MAB245
  • Human IFN-alpha / beta  R1 Antibody
    Name: Satbir Thakur
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: THP-1 cell lysate and Human
    Verified Customer | Posted 09/30/2020
    western blot analysis
    Human IFN‑ alpha / beta  R1 Antibody MAB245
  • Human IFN-alpha / beta  R1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    Human IFN‑ alpha / beta  R1 Antibody MAB245
  • Human IFN-alpha / beta  R1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018

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FAQs

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail