Human IFN-alpha / beta  R1 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB245F
Human IFN-alpha / beta  R1 Fluorescein-conjugated Antibody in Flow Cytometry
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Product Details
Citations (3)
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Human IFN-alpha / beta  R1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) IFN‑ gamma  R1, rhIFN-gamma R2, recombinant mouse IFN-alpha / beta  R2, rhIL-10 R beta, rhIL-10 R alpha, or rhIL-20 R beta.
Source
Monoclonal Mouse IgG1 Clone # 85228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta  R1
Lys28-Lys436
Accession # AAA52730
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IFN‑ alpha / beta  R1 in U937 Human Cell Line by Flow Cytometry. View Larger

Detection of IFN‑ alpha / beta R1 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human IFN-a/ beta R1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB245F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name
Interferon alpha/beta Receptor 1
Entrez Gene IDs
3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

Product Datasheets

Citations for Human IFN-alpha / beta  R1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
    Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R
    PLoS Pathog, 2013;9(4):e1003316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Interferon-alpha triggers B cell effector 1 (Be1) commitment.
    Authors: de Goer de Herve MG, Durali D, Dembele B, Giuliani M, Tran TA, Azzarone B, Eid P, Tardieu M, Delfraissy JF, Taoufik Y
    PLoS ONE, 2011;6(4):e19366.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human metapneumovirus inhibits IFN-alpha signaling through inhibition of STAT1 phosphorylation.
    Authors: Dinwiddie DL, Harrod KS
    Am. J. Respir. Cell Mol. Biol., 2008;38(6):661-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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