Human IFN-alpha / beta  R1 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB245F
Detection of IFN‑ alpha / beta  R1 in U937 Human Cell Line by Flow Cytometry.
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Citations (3)
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Human IFN-alpha / beta  R1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) IFN‑ gamma  R1, rhIFN-gamma R2, recombinant mouse IFN-alpha / beta  R2, rhIL-10 R beta, rhIL-10 R alpha, or rhIL-20 R beta.
Source
Monoclonal Mouse IgG1 Clone # 85228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta  R1
Lys28-Lys436
Accession # AAA52730
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IFN-a/ beta  R1 antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IFN‑ alpha / beta R1 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human IFN-a/ beta R1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB245F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name
Interferon alpha/beta Receptor 1
Entrez Gene IDs
3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

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Product Specific Notices

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source.

Citations for Human IFN-alpha / beta  R1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
    Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R
    PLoS Pathog, 2013-04-18;9(4):e1003316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Interferon-alpha triggers B cell effector 1 (Be1) commitment.
    Authors: de Goer de Herve MG, Durali D, Dembele B, Giuliani M, Tran TA, Azzarone B, Eid P, Tardieu M, Delfraissy JF, Taoufik Y
    PLoS ONE, 2011-04-29;6(4):e19366.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human metapneumovirus inhibits IFN-alpha signaling through inhibition of STAT1 phosphorylation.
    Authors: Dinwiddie DL, Harrod KS
    Am. J. Respir. Cell Mol. Biol., 2008-01-24;38(6):661-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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