Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
Ala22-Gln247
Accession # P38484

Specificity

Detects human IFN-gamma  R2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human IFN‑ gamma  R1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IFN‑ gamma  R2 APC‑conjugated Antibody

Detection of IFN-? R2 antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of IFN‑ gamma R2 in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Goat Anti-Human IFN-gamma R2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB773A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IFN‑ gamma  R2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood granulocytes

Reviewed Applications

Read 1 review rated 3 using FAB773A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IFN-gamma R2

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma  R2 is required for signal transduction but does not bind IFN-gamma by itself.

Long Name

Interferon gamma Receptor 2

Alternate Names

AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1

Entrez Gene IDs

3460 (Human); 15980 (Mouse)

Gene Symbol

IFNGR2

UniProt

Additional IFN-gamma R2 Products

Product Documents for Human IFN‑ gamma  R2 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IFN‑ gamma  R2 APC‑conjugated Antibody

For research use only

Citations for Human IFN‑ gamma  R2 APC‑conjugated Antibody

Customer Reviews for Human IFN‑ gamma  R2 APC‑conjugated Antibody (1)

3 out of 5
1 Customer Rating
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  • Human IFN-gamma  R2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 12/11/2016
    Cell surface staining of monocytes from freshly isolated PBMC with APC-hIFNgR2 (black line). In grey is isotype control. Antibody used at 1:5 dilution. Cells fixed with BD Fix/Perm before acquiring.
    Staining is acceptable but have to be used at very high concentrations.
    Human IFN‑ gamma  R2 APC‑conjugated Antibody FAB773A

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