Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma R2 is required for signal transduction but does not bind IFN-gamma by itself.
Human IFN‑ gamma R2 Biotinylated Antibody
R&D Systems | Catalog # BAF773
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
Ala22-Gln247
Accession # P38484
Ala22-Gln247
Accession # P38484
Specificity
Detects human IFN‑ gamma R2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IFN-gamma RI and rhIL‑10 R beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IFN‑ gamma R2 Biotinylated Antibody
Detection of IFN‑ gamma R2 in THP‑1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Goat Anti-Human IFN-gamma R2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF773, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).Applications for Human IFN‑ gamma R2 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line
Sample: THP‑1 human acute monocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human IFN-gamma R2
Sample: Recombinant Human IFN-gamma R2
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IFN-gamma R2
Long Name
Interferon gamma Receptor 2
Alternate Names
AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1
Gene Symbol
IFNGR2
UniProt
Additional IFN-gamma R2 Products
Product Documents for Human IFN‑ gamma R2 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IFN‑ gamma R2 Biotinylated Antibody
For research use only
Citations for Human IFN‑ gamma R2 Biotinylated Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
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- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Th1 Differentiation Pathway
