Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma R2 is required for signal transduction but does not bind IFN-gamma by itself.
Human IFN-gamma R2 Antibody [Biotin]
R&D Systems | Catalog # BAF773
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
Ala22-Gln247
Accession # P38484
Ala22-Gln247
Accession # P38484
Specificity
Detects human IFN‑ gamma R2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IFN-gamma RI and rhIL‑10 R beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IFN-gamma R2 Antibody [Biotin]
Detection of IFN‑ gamma R2 in THP‑1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Goat Anti-Human IFN-gamma R2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF773, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).Applications for Human IFN-gamma R2 Antibody [Biotin]
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line
Sample: THP‑1 human acute monocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human IFN-gamma R2
Sample: Recombinant Human IFN-gamma R2
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IFN-gamma R2
Long Name
Interferon gamma Receptor 2
Alternate Names
AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1
Gene Symbol
IFNGR2
UniProt
Additional IFN-gamma R2 Products
Product Documents for Human IFN-gamma R2 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IFN-gamma R2 Antibody [Biotin]
For research use only
Citations for Human IFN-gamma R2 Antibody [Biotin]
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
