Human IL-10 R alpha Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2742R-100UG
Human IL-10 R alpha Alexa Fluor® 647-conjugated Antibody in Flow Cytometry
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Human IL-10 R alpha Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IL-10 R alpha or recombinant human (rh) IL-10 R beta is observed. In Western blots, no cross-reactivity with rhIL-10 R beta is observed.
Source
Monoclonal Mouse IgG2B Clone # 714212
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10 R alpha
His22-Asn235
Accession # Q13651
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL-10 R alpha  in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of IL-10 R alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human IL-10 R alpha Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB2742R) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041R) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody(Catalog # FAB4867P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10. Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Long Name
Interleukin 10 Receptor alpha
Entrez Gene IDs
3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names
CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha

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