Human IL-10 R alpha Alexa Fluor® 647-conjugated Antibody

FAB2742R Datasheet / COA / SDS

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FAB2742R-100UG

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Human IL-10 R alpha Alexa Fluor® 647-conjugated Antibody in Data

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Human IL-10 R alpha Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-10 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IL-10 R alpha or recombinant human (rh) IL-10 R beta is observed. In Western blots, no cross-reactivity with rhIL-10 R beta is observed.

Source

Monoclonal Mouse IgG_2B Clone # 714212

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10 R alpha
His22-Asn235
Accession # Q13651

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of IL-10 R alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human IL-10 Ra Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB2742R) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041R) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody(Catalog # FAB4867P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10. Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Long Name

Interleukin 10 Receptor alpha

Entrez Gene IDs

3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)

Alternate Names

CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha