Human IL-10 R beta Alexa Fluor® 700-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human IL-10 R beta  in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑10 R alpha, rhIFN-gamma  RI, and rhIFN-gamma  R2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 90220
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
    Met20-Ser220
    Accession # Q08334
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of IL‑10 R beta in Human PBMC Monocytes by Flow Cytometry Human PBMC monocytes were stained with Mouse Anti-Human IL‑10 R beta Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB874N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-10 R beta

IL-10 R beta, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

  • References:
    1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
    2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
    3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
    4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
    5. Lutfalla, G. et al. (1993) Genomics 16:366.
    6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  • Long Name:
    Interleukin 10 Receptor beta
  • Entrez Gene IDs:
    3588 (Human); 16155 (Mouse)
  • Alternate Names:
    CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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