Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 90220
View all IL-10 R beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
Met20-Ser220
Accession # Q08334

Specificity

Detects human IL-10 R beta  in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑10 R alpha, rhIFN-gamma  RI, and rhIFN-gamma  R2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑10 R beta Antibody

IL‑10 Inhibition of IL‑1 beta secre-tion and Neutralization by Human IL‑10 R beta Antibody.

IL‑10 Inhibition of IL‑1 beta secre-tion and Neutralization by Human IL‑10 R beta Antibody.

Recombinant Human IL-10 (Catalog # 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta /IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secre-tion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 R beta Monoclonal Antibody (Catalog # MAB874). The ND50 is typically 0.5-2 µg/mL in the presence of LPS (0.25 ng/mL).
Detection of IL-10 R beta antibody in Human PBMC Monocytes antibody by Flow Cytometry

Detection of IL‑10 R beta in Human PBMC Monocytes by Flow Cytometry

Human PBMC monocytes were stained with Mouse Anti-Human IL-10 R beta Monoclonal Antibody (Catalog # MAB874, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL‑10 R beta Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC monocytes

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑10 R beta Fc Chimera (Catalog # 874-RB)

Neutralization

Measured by its ability to neutralize IL‑10 R beta -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.

Reviewed Applications

Read 1 review rated 3 using MAB874 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10 R beta

IL-10 R beta, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

References

  1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
  3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
  5. Lutfalla, G. et al. (1993) Genomics 16:366.
  6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.

Long Name

Interleukin 10 Receptor beta

Alternate Names

CDw210b, CRFB4, D21S58, IL-10R2, IL-10Rb, IL10R beta, IL10RB

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Gene Symbol

IL10RB

UniProt

Q08334

Additional IL-10 R beta Products

Product Documents for Human IL‑10 R beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑10 R beta Antibody

For research use only

Citations for Human IL‑10 R beta Antibody

Customer Reviews for Human IL‑10 R beta Antibody (1)

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  • Human IL-10 R beta Antibody
    Name: Jan Martinek
    Application: Immunohistochemistry-Frozen
    Sample Tested: Human Tonsil tissue
    Species: Human
    Verified Customer | Posted 01/15/2021
    Human tonsil stained for IL10Rb (red) and counterstained with DAPI (blue)
    Acetone fixation
    Human IL‑10 R beta Antibody MAB874

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail