Human IL-10 R beta Antibody

Catalog # Availability Size / Price Qty
MAB874-SP
MAB874-500
MAB874-100
IL‑10 Inhibition of IL‑1 beta  secre-tion and Neutralization by Human IL‑10 R beta  Antibody.
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Product Details
Citations (5)
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Human IL-10 R beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 R beta  in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑10 R alpha, rhIFN-gamma  RI, and rhIFN-gamma  R2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 90220
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
Met20-Ser220
Accession # Q08334
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑10 R beta Fc Chimera (Catalog # 874-RB)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑10 R beta -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑10 Inhibition of IL‑1 beta  secre-tion and Neutralization by Human IL‑10 R beta  Antibody. View Larger

IL‑10 Inhibition of IL‑1 beta secre-tion and Neutralization by Human IL‑10 R beta Antibody. Recombinant Human IL-10 (Catalog # 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta /IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secre-tion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 R beta Monoclonal Antibody (Catalog # MAB874). The ND50 is typically 0.5-2 µg/mL in the presence of LPS (0.25 ng/mL).

Flow Cytometry Detection of IL-10 R beta antibody in Human PBMC Monocytes antibody by Flow Cytometry View Larger

Detection of IL‑10 R beta in Human PBMC Monocytes by Flow Cytometry Human PBMC monocytes were stained with Mouse Anti-Human IL-10 R beta Monoclonal Antibody (Catalog # MAB874, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R beta

IL-10 R beta, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

References
  1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
  3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
  5. Lutfalla, G. et al. (1993) Genomics 16:366.
  6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Long Name
Interleukin 10 Receptor beta
Entrez Gene IDs
3588 (Human); 16155 (Mouse)
Alternate Names
CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4

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Citations for Human IL-10 R beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
    Authors: AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo
    J. Immunol., 2017-10-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Immune Cell Profiling of IFN-? Response Shows pDCs Express Highest Level of IFN-?R1 and Are Directly Responsive Via the JAK-STAT Pathway
    J Interferon Cytokine Res, 2016-09-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL22/IL-22R pathway induces cell survival in human glioblastoma cells.
    Authors: Akil H, Abbaci A, Lalloue F, Bessette B, Costes L, Domballe L, Charreau S, Guilloteau K, Karayan-Tapon L, Bernard F, Morel F, Jauberteau M, Lecron J
    PLoS ONE, 2015-03-20;10(3):e0119872.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  4. Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
    Authors: Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C
    Eur. J. Immunol., 2008-12-01;38(12):3365-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
    Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
    J. Virol., 2008-01-23;82(7):3736-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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