Human IL-15R alpha APC-conjugated Antibody

R&D Systems | Catalog # FAB1471A

R&D Systems
Discontinued Product
FAB1471A has been discontinued. View all IL-15R alpha products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 151303
View all IL-15R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑15 R alpha
Ile31-Thr172
Accession # EAW86418

Specificity

Detects human IL‑15 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rhIL‑2 R beta, rhIL‑2 R gamma, or rmIL‑15 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IL-15R alpha APC-conjugated Antibody

Detection of IL-15 Ra antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑15 R alpha in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PHA overnight were stained with Mouse Anti-Human IL-15 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1471A) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-15R alpha APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA

Reviewed Applications

Read 1 review rated 4 using FAB1471A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References

  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Gene Symbol

IL15RA

UniProt

EAW86418

Additional IL-15R alpha Products

Product Documents for Human IL-15R alpha APC-conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human IL-15R alpha APC-conjugated Antibody

For research use only

Citations for Human IL-15R alpha APC-conjugated Antibody

Customer Reviews for Human IL-15R alpha APC-conjugated Antibody (1)

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  • Human IL-15 R alpha APC-conjugated Antibody
    Name: Preeti Bharaj
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 12/16/2017
    Human IL-15R alpha APC-conjugated Antibody FAB1471A

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