Human IL-15R alpha PE-conjugated Antibody
Human IL-15R alpha PE-conjugated Antibody Summary
Ile31-Thr172
Accession # EAW86418
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Detection of IL‑15 R alpha in Human CD4+PBMCs by Flow Cytometry. Human CD4+peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL PHA for 5 days were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human IL-15 Ra PE-conjugated Monoclonal Antibody (Catalog # FAB1471P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-15R alpha
Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.
- Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
- Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
- Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
- Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
Product Datasheets
Citations for Human IL-15R alpha PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Rheumatoid arthritis bone marrow environment supports Th17 response
Authors: E Kuca-Warna, W Kurowska, M Prochorec-, A Radzikowsk, T Burakowski, U Skalska, M Massalska, M Pleba?czyk, B Ma?dyk-Now, I S?owi?ska, R Gasik, W Ma?li?ski
Arthritis Res. Ther., 2017;19(1):274.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Rheumatoid arthritis bone marrow environment supports Th17 response.
Authors: Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski
Arthritis Research & Therapy, 2017;0(0):1478-6362.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
J Immunol, 2014;192(1):123-35.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
