Human IL-18/IL-1F4 Antibody

Catalog # Availability Size / Price Qty
D043-3
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human IL-18/IL-1F4 Antibody Summary

Species Reactivity
Human
Specificity
This antibody reacts with human IL-18 on Western blotting.
Source
Monoclonal Mouse IgG1 Clone # 25-2G
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human IL-18/IL-1F4
Formulation
This antibody is in lyophilized form. After reconstitution, IgG concentration should be 1 mg/mL in PBS (pH 7.2)/1% sucrose. Contains no preservatives.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 μg/mL for chemiluminescencedetection system
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Prepare a stock solution by dissolving the lyophilized antibody with 100 μL of distilled water.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for one year from the date of purchase when stored at 4°C. After reconstitution, avoid repeated freezing and thawing. For storage, prepare appropriate aliquots and freeze at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References
  1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
  2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
  3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
  4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
  5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
  6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
  7. Okamura, H., et al. Nature 378, 88-91 (1995)
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

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Citations for Human IL-18/IL-1F4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity
    Authors: J Saetang, N Roongsawan, S Sangkhatha, SP Voravuthik, N Sangkaew, N Prompat, T Srichana, V Tipmanee
    PeerJ, 2022-07-05;10(0):e13626.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
    Authors: Y Fan, X Zhang, L Yang, J Wang, Y Hu, A Bian, J Liu, J Ma
    Mol Med Rep, 2017-08-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.
    Authors: Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.
    Authors: Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB
    Exp. Dermatol., 2004-12-01;13(12):764-72.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
    Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
    Arthritis Rheum., 2003-02-01;48(2):334-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human IL-18/IL-1F4 Antibody

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Human IL-18/IL-1F4 Antibody
By Anonymous on 10/31/2017
Application: ELISA Sample Tested: CHO Chinese hamster ovary cell line Species: Hamster