Human IL-19 Quantikine ELISA Kit

(3 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL), Human Milk (50 uL)
  • Sensitivity
    12.2 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
  • Specificity
    Natural and recombinant human IL-19
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC24, Quantikine Immunoassay Control Group 9 - Please Inquire
Product Summary
The Quantikine Human IL-19 immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-19 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human IL-19 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-19 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-19.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean29280515862697391478
Standard Deviation10.31941.82256.8103
CV%3.52.42.68.27.77

Recovery

The recovery of human IL-19 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-104
EDTA Plasma (n=4) 94 85-102
Heparin Plasma (n=4) 93 87-99
Serum (n=4) 93 87-97
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-19 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-19 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-19
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. IL-19 contains seven helices and is secreted as a 35 kDa glycosylated monomer. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24.
    • Long Name
      Interleukin 19
    • Entrez Gene IDs
      29949 (Human); 329244 (Mouse);
    • Alternate Names
      IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    3 Citations: Showing 1 - 3
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    Species
    Sample Type
    1. Interleukin-19 impairment in active Crohn's disease patients.
      Authors: Canto E, Garcia Planella E, Zamora-Atenza C, Nieto J, Gordillo J, Ortiz M, Meton I, Serrano E, Vegas E, Garcia-Bosch O, Juarez C, Vidal S
      PLoS ONE, 2014;9(4):e93910.
      Species: Human
      Sample Type: Serum
    2. Crimean Congo hemorrhagic fever virus infects human monocyte-derived dendritic cells.
      Authors: Connolly-Andersen AM, Douagi I, Kraus AA, Mirazimi A
      Virology, 2009;390(2):157-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
      Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
      Eur. J. Immunol., 2009;39(12):3570-81.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Group 9

    Ctrl QC24
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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