Mature human and mouse IL-20 is a 152 amino acid protein that is not likely glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor-expressing cells.
Human IL-20 Quantikine ELISA Kit
R&D Systems | Catalog # DL200
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
16.6 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human IL-20 Quantikine ELISA Kit
The Quantikine Human IL-20 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-20 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-20 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-20 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-20.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human IL-20
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 461 | 984 | 1983 | 491 | 961 | 1882 |
| Standard Deviation | 26.1 | 70.4 | 159 | 39.8 | 74.2 | 106 |
| CV% | 5.7 | 7.2 | 8.0 | 8.1 | 7.7 | 5.6 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 522 | 1143 | 2183 | 518 | 1079 | 2092 |
| Standard Deviation | 32.2 | 103 | 208 | 46.4 | 61.8 | 101 |
| CV% | 6.2 | 9.0 | 9.5 | 9.0 | 5.7 | 4.8 |
Recovery for Human IL-20 Quantikine ELISA Kit
The recovery of IL-20 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 96-111 |
| EDTA Plasma (n=4) | 107 | 103-113 |
| Heparin Plasma (n=4) | 101 | 86-108 |
| Serum (n=4) | 100 | 91-109 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-20 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-20 Quantikine ELISA Kit
Human IL-20 ELISA Cell Culture Supernate Standard Curve
Human IL-20 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-20
Long Name
Interleukin 20
Alternate Names
IL20
Gene Symbol
IL20
Additional IL-20 Products
Product Documents for Human IL-20 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL-20 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human IL-20 Quantikine ELISA Kit
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Protocols
View specific protocols for Human IL-20 Quantikine ELISA Kit (DL200):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
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