Human IL-34 Biotinylated Antibody

Catalog #: BAM52652 Datasheet / COA / SDS

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BAM52652

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-34 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-34 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse IL-34 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 578401

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-34
Asn21-Pro242
Accession # NP_689669

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human IL-34 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human IL‑34 Antibody (Catalog # MAB52651)

Standard: Recombinant Human IL-34 Protein (Catalog # 5265-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. The mature chain contains one potential site for N-linked glycosylation. IL-34 is widely expressed, but it is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). Functionally, IL-34 stimulates monocyte viability.

Long Name

Interleukin 34

Entrez Gene IDs

146433 (Human); 76527 (Mouse)

Alternate Names

C16orf77; IL34; IL-34; interleukin 34