Human IL-34 Quantikine ELISA Kit

In alignment with Bio-Techne’s commitment to sustainability and Go Green initiatives, we will no longer pack paper kit booklets in the following immunoassay kits:

  • Quantikine® RUO ELISA Kits                                   
  • Quantikine® HS ELISA Kits
  • QuantiGloELISA Kits
  • QuicKitELISA Kits
  • Fluorokine Kits
  • Parameter Assay Kits
  • ELISpot Kits
Catalog # Availability Size / Price Qty
D3400
Control Products Available
Human IL-34 ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (4)
FAQs
Supplemental Products
Reviews

Human IL-34 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Human Milk (13 uL)
Sensitivity
3.06 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)
Specificity
Natural and recombinant human IL-34
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-34 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-34 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-34 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-34. Results obtained using natural human IL-34 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-34.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 212 571 1174 215 597 1206
Standard Deviation 15.5 14.4 21.2 12.8 35.4 50
CV% 7.3 2.5 1.8 6 5.9 4.1

Recovery

The recovery of human IL-34 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 97 85-104
EDTA Plasma (n=4) 97 90-102
Heparin Plasma (n=4) 97 88-105
Serum (n=4) 98 90-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-34 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-34 ELISA Linearity

Scientific Data

Human IL-34 ELISA Standard Curve

Product Datasheets

You must select a language.

x

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level.

IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.

Long Name:
Interleukin 34
Entrez Gene IDs:
146433 (Human); 76527 (Mouse)
Alternate Names:
C16orf77; IL34; IL-34; interleukin 34
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-34 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. CSF1R signaling is a regulator of pathogenesis in progressive MS
    Authors: N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim
    Cell Death Dis, 2020-10-23;11(10):904.
    Species: Human
    Sample Types: CSF
  2. Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice
    Authors: S Mathews, A Branch Woo, I Katano, E Makarov, MB Thomas, HE Gendelman, LY Poluektova, M Ito, S Gorantla
    Mol Neurodegener, 2019-03-05;14(1):12.
    Species: Humanized Mouse
    Sample Types: Plasma
  3. Prognostic Significance of Interleukin-34 (IL-34) in Patients With Chronic Heart Failure With or Without Renal Insufficiency
    Authors: R Tao, Q Fan, H Zhang, H Xie, L Lu, G Gu, F Wang, R Xi, J Hu, Q Chen, W Niu, W Shen, R Zhang, X Yan
    J Am Heart Assoc, 2017-04-01;6(4):.
    Species: Human
    Sample Types: Serum
  4. IL-34 is associated with the presence and severity of renal dysfunction and coronary artery disease in patients with heart failure
    Sci Rep, 2016-12-16;6(0):39324.
    Species: Human
    Sample Types: Serum

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Loading...

Reviews for Human IL-34 Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human IL-34 Quantikine ELISA Kit and earn rewards!

Have you used Human IL-34 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review