Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 647-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISA.
  • Source
    Monoclonal Mouse IgG1 Clone # 700838
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    Human myeloid-derived suppressor cells (MDSCs) treated with Recombinant Human IL‑6 (Catalog # 206-IL) and Recombinant Human GM‑CSF (Catalog # 215-GM) were fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Indoleamine 2,3-dioxygenase/IDO
Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).
  • References:
    1. Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
    2. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
    3. Xu, H. et al. (2008) Immunol. Lett. 121:1.
    4. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
    5. Curti, A. et al. (2009) Blood 113:2394.
  • Entrez Gene IDs:
    3620 (Human); 15930 (Mouse)
  • Alternate Names:
    3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase
Related Research Areas

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