Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6030R-100UG
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Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISA.
Source
Monoclonal Mouse IgG1 Clone # 700838
Immunogen
E. coli-derived recombinant human Indoleamine 2,3-dioxygenase/IDO
Ala2-Gly403
Accession # P14902
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Human myeloid-derived suppressor cells (MDSCs) treated with Recombinant Human IL‑6 (Catalog # 206-IL) and Recombinant Human GM‑CSF (Catalog # 215-GM) were fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).

References
  1. Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
  2. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
  3. Xu, H. et al. (2008) Immunol. Lett. 121:1.
  4. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
  5. Curti, A. et al. (2009) Blood 113:2394.
Entrez Gene IDs
3620 (Human); 15930 (Mouse)
Alternate Names
3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase

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