Human Integrin  alpha 2b/CD41 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB76161V Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB76161V-100UG
Product Details
FAQs
Supplemental Products
Reviews

Human Integrin  alpha 2b/CD41 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects Human Integrin alpha 2b beta 3 heterodimer in direct ELISAs. In direct ELISAs, no cross-reactivity with recomabint human (rh) Integrin alpha 2b, rhIntegrin alpha 5, rhIntegrin alpha 5 beta 6, rhIntegrin alpha 8 beta 1, rhIntegrin beta 1, rhIntegrin beta 2, rhIntegrin beta 3, rhIntegrin beta 5, rhIntegrin beta 7, and recombinant mouse Integrin alpha 2b beta 3.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2530A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived heterodimer of Human Integrin alpha 2b (Leu32-Arg993, Accession P08514) and Human Integrin beta 3 (Gly27-Asp718, Accession P05106)
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood platelets

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 2b/CD41

Integrin alpha 2b beta 3 (also alpha IIb beta 3 or GPIIbIIIa) is the only alpha 2b integrin and shares the beta 3 subunit only with alpha V beta 3 (1‑3). It is the non‑covalent heterodimer of type I transmembrane subunits, alpha 2b/CD41 (present as a disulfide‑linked complex of 114 kDa heavy and 22 kDa light chains) and 93 kDa beta 3/CD61 (1‑3). It is the most abundant integrin expressed by megakaryocytes and platelets, both on the surface and within alpha granules (1, 2). Deficiencies of alpha 2b beta 3 produce Glanzmann thrombasthenia, a potentially serious bleeding disorder (4). In its constitutively inactive state, alpha 2b beta 3 is flexed within the extracellular domains. Activation, either by intracellular signaling or by Mg2+ or Mn2+ binding, extends the integrin to expose the ligand binding site created by interaction of the beta 3 vWFA domain with the alpha 2b  beta ‑propeller structure (1). The 962 aa human alpha 2b ECD shares 78‑83% aa sequence identity with mouse, rat, canine, equine and porcine  alpha 2b while the 685 aa human  beta 3 ECD shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and porcine beta 3. It is unclear whether splice variants of beta 3 that differ in the cytoplasmic domain are expressed significantly in platelets (5‑7). However, platelet expression of a beta 3 splice variant that produces a soluble 60 kDa beta 3 isoform, and an alpha 2b isoform lacking aa 948‑982, have been reported (7, 8). Active cell surface alpha 2b beta 3 adheres to fibrinogen, mediating platelet/platelet interactions that initiate a cascade of platelet activation and aggregation, extracellular matrix adhesion, formation of thrombi and clot retraction (1). It also binds matrix proteins that have an RGD motif, including fibronectin, plasminogen, prothrombin, thrombospondin and vitronectin (1, 2). Targeting of alpha 2b beta 3 by therapeutic antibodies or small molecules can inhibit formation of thrombi in patients with acute coronary syndrome, and potentially inhibits tumor angiogenesis and metastasis by blocking interaction of platelet alpha 2b beta 3 with tumor cells (1, 9).

References
  1. Kasirer-Friede, A. et al. (2007) Immunol. Rev. 218:247.
  2. Poncz, M. et al. (1987) J. Biol. Chem. 262:8476.
  3. Fitzgerald, L.A. et al. (1987) J. Biol. Chem. 262:3936.
  4. Franchini, M. et al. (2010) Clin. Chim. Acta 411:1.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. van Kuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
  8. Bray, P.F. et al. (1990) J. Biol. Chem. 265:9587.
  9. Erpenbeck, L. and M.P. Schon (2010) Blood 115:3427.
Entrez Gene IDs
3674 (Human); 16399 (Mouse); 29350 (Rat); 102119413 (Cynomolgus Monkey)
Alternate Names
CD41 antigen; CD41; CD41BHPA3; GP2B; GP2Bintegrin alpha-IIb; GPalpha IIb; GPIIb; GTA; HPA3; Integrin alpha 2b; integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41); integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B); ITGA2b; ITGAB; platelet fibrinogen receptor, alpha subunit; Platelet membrane glycoprotein IIb; platelet-specific antigen BAK

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Integrin  alpha 2b/CD41 Alexa Fluor® 405-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Integrin  alpha 2b/CD41 Alexa Fluor® 405-conjugated Antibody and earn rewards!

Have you used Human Integrin  alpha 2b/CD41 Alexa Fluor® 405-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review