Human Integrin  alpha 8 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6194A
Detection of Integrin  alpha 8 in H4 Human Cell Line by Flow Cytometry.
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Human Integrin  alpha 8 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 8 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 481709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Integrin alpha 8
Glu610-Leu800
Accession # P53708
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a8 antibody in H4 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 8 in H4 Human Cell Line by Flow Cytometry. H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin a8 APC-conjugated Monoclonal Antibody (Catalog # FAB6194A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 8

Integrin alpha 8 is a 170-200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. Integrin alpha 8/ beta 1 promotes both cell adhesion and survival, and is known to bind to fibronectin, LAP-TGF beta 1 and Nephronectin. It is principally expressed in smooth muscle cells.  Human alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 974 aa extracellular domain (ECD) (aa 39-1012) and a 30 aa cytoplasmic domain. In the ECD, human alpha 8 shares 90% aa sequence identity with mouse alpha 8 protein.

Entrez Gene IDs
8516 (Human); 241226 (Mouse); 84381 (Rat)
Alternate Names
Integrin alpha 8; integrin alpha-8; integrin, alpha 8; ITGA8

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