Detection of Integrin alpha 8 in H4 Human Cell Line by Flow Cytometry.
H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin alpha 8 APC‑conjugated Monoclonal Antibody (Catalog # FAB6194A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 8
Integrin alpha 8 is a 170-200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. Integrin alpha 8/ beta 1 promotes both cell adhesion and survival, and is known to bind to fibronectin, LAP-TGF beta 1 and Nephronectin. It is principally expressed in smooth muscle cells. Human alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 974 aa extracellular domain (ECD) (aa 39-1012) and a 30 aa cytoplasmic domain. In the ECD, human alpha 8 shares 90% aa sequence identity with mouse alpha 8 protein.
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