Human Integrin  alpha 8 Antibody

  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha 8 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 481709
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Integrin alpha 8
    Glu610-Leu800
    Accession # P53708
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  alpha 8 in H4 Human Cell Line by Flow Cytometry. H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin  alpha 8 Monoclonal Antibody (Catalog # MAB6194, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
Integrin  alpha 8 in H4 Human Cell Line. Integrin  alpha 8 was detected in immersion fixed H4 human neuroglioma cell line using Mouse Anti-Human Integrin  alpha 8 Monoclonal Antibody (Catalog # MAB6194) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Integrin alpha 8 is a 170-200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. Integrin alpha 8/ beta 1 promotes both cell adhesion and survival, and is known to bind to fibronectin, LAP-TGF beta 1 and Nephronectin. It is principally expressed in smooth muscle cells.  Human alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 974 aa extracellular domain (ECD) (aa 39-1012) and a 30 aa cytoplasmic domain. In the ECD, human alpha 8 shares 90% aa sequence identity with mouse alpha 8 protein.
  • Entrez Gene IDs:
    8516 (Human); 241226 (Mouse); 84381 (Rat)
  • Alternate Names:
    Integrin alpha 8; integrin alpha-8; integrin, alpha 8; ITGA8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. 9-cis-retinoic Acid and troglitazone impacts cellular adhesion, proliferation, and integrin expression in K562 cells.
    Authors: Hanson A, Gambill J, Phomakay V, Staten C, Kelley M
    PLoS ONE, 2014;9(3):e93005.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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