Detects human Integrin beta 1/CD29. Reacts with calcium-dependent epitopes on the human Integrin beta 1 subunit. This antibody activates Integrin beta 1‑dependent binding to extracellular matrix substrates. The use of binding buffer containing 1 mM Ca+2 is recommended.
Monoclonal Mouse IgG1 Clone # P4G11
Protein A or G purified from hybridoma culture supernatant
U937 human histiocytic lymphoma cell line
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Integrin beta 1/CD29 in HeLa Human Cell Line. Integrin beta 1/CD29 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17782) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as CD29, associates with multiple
distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad
tissue distribution with the exception of erythrocytes.
Wayner, E.A. et al. (1993) J. Cell Biol. 121:1141.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.