Human Integrin beta 1/CD29 Antibody

Name: Human Integrin beta 1/CD29 Antibody
Brand: R&D Systems
SKU: MAB17782

R&D Systems | Catalog # MAB17782

Submit a review

Citations (2)

Product Documents

Discontinued Product

MAB17782 has been discontinued. An alternative/replacement product is available: MAB17781. View all Integrin beta 1/CD29 products.

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Adhesion Activation, CyTOF-ready

Cited:

Neutralization, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # P4G11

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

U937 human histiocytic lymphoma cell line

Specificity

Detects human Integrin beta 1/CD29. Reacts with calcium-dependent epitopes on the human Integrin beta 1 subunit. This antibody activates Integrin beta 1‑dependent binding to extracellular matrix substrates. The use of binding buffer containing 1 mM Ca+

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin beta 1/CD29 Antibody

Integrin beta 1/CD29 in HeLa Human Cell Line.

Integrin beta 1/CD29 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17782) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin beta 1/CD29 Antibody

Application

Recommended Usage

Adhesion Activation

This antibody was shown to activate Integrin beta 1-mediated cell adhesion. Wayner, E.A. et al. (1993) J. Cell Biol. 121:1141. This application was not tested by R&D Systems.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells

Immunocytochemistry

Qin, L. et al. (2003) Biol. Reprod. 69:563 and Kemp, B. et al. (2002) Histochem. Cell. Biol. 117:401.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Integrin beta 1/CD29, see our available Western blot detection antibodies

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.