Human Integrin  beta 1/CD29 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin beta 1/CD29. Reacts with calcium-dependent epitopes on the human Integrin beta 1 subunit. This antibody activates Integrin  beta 1‑dependent binding to extracellular matrix substrates. The use of binding buffer containing 1 mM Ca+2 is recommended.
  • Source
    Monoclonal Mouse IgG1 Clone # P4G11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    U937 human histiocytic lymphoma cell line
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Human peripheral blood mononuclear cells
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Integrin  beta 1/CD29, see our available Western blot detection antibodies
  • Adhesion Activation
    This antibody was shown to activate Integrin beta 1-mediated cell adhesion. Wayner, E.A. et al. (1993) J. Cell Biol. 121:1141. This application was not tested by R&D Systems.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry

    Qin, L. et al. (2003) Biol. Reprod. 69:563 and Kemp, B. et al. (2002) Histochem. Cell. Biol. 117:401.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Integrin  beta 1/CD29 in HeLa Human Cell Line. Integrin  beta 1/CD29 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17782) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.
  • References:
    1. Wayner, E.A. et al. (1993) J. Cell Biol. 121:1141.
  • Entrez Gene IDs:
    3688 (Human); 16412 (Mouse)
  • Alternate Names:
    CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. The tetraspanin CD9 modulates epidermal growth factor receptor signaling in cancer cells.
    Authors: Murayama Y, Shinomura Y, Oritani K, Miyagawa J, Yoshida H, Nishida M, Katsube F, Shiraga M, Miyazaki T, Nakamoto T, Tsutsui S, Tamura S, Higashiyama S, Shimomura I, Hayashi N
    J. Cell. Physiol., 2008;216(1):135-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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ExactaChIP Chromatin IP Buffer Panel

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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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