Human Integrin  beta 1/CD29 Antibody

  (15 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (15)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin beta 1/CD29.
  • Source
    Monoclonal Mouse IgG1 Clone # P5D2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human skin keratinocytes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoprecipitation

    Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
    Joneckis, C.C. et al. (1993) Blood 82:3548.

  • Blockade of Receptor-ligand Interaction
    Mukhopadhyay, N.K. et al. (2004) Ann. Thorac. Surg. 78:450.
    Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
Integrin  beta 1/CD29 in human PBMCs. Integrin  beta 1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.
  • References:
    1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
    2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.
  • Entrez Gene IDs:
    3688 (Human); 16412 (Mouse)
  • Alternate Names:
    CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Doxorubicin resistance in breast cancer cells is mediated by extracellular matrix proteins
    Authors: CJ Lovitt, TB Shelper, VM Avery
    BMC Cancer, 2018;18(1):41.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin ?1 to Promote Embryo-tubal Attachment
    Authors: SF Ahmad, JK Brown, LL Campbell, M Koscielnia, C Oliver, N Wheelhouse, G Entrican, S McFee, GS Wills, MO McClure, PJ Horner, S Gaikoumelo, KF Lee, HOD Critchley, WC Duncan, AW Horne
    EBioMedicine, 2018;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Fibronectin-guided migration of carcinoma collectives
    Authors: S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg
    Nat Commun, 2017;8(0):14105.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016;6(0):34310.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells
    Authors: Rickard J Storm
    J. Virol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
    Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
    Cell Commun Signal, 2014;12(0):36.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.
    Authors: Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C
    J Exp Med, 2014;211(13):2549-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  8. The alpha-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in beta3 integrins.
    Authors: Rui X, Mehrbod M, Van Agthoven J, Anand S, Xiong J, Mofrad M, Arnaout M
    J Biol Chem, 2014;289(33):23256-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Cultivation of corneal endothelial cells on a pericellular matrix prepared from human decidua-derived mesenchymal cells.
    Authors: Numata R, Okumura N, Nakahara M, Ueno M, Kinoshita S, Kanematsu D, Kanemura Y, Sasai Y, Koizumi N
    PLoS ONE, 2014;9(2):e88169.
  10. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.
    Authors: Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, Paul A, Esposito E, Gridley S, Leitinger B, Naegle K, Huang P
    Biochem J, 2013;454(3):501-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly.
    Authors: Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM
    PLoS ONE, 2012;7(3):e33915.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection.
    Authors: Shannon-Lowe C, Rowe M
    PLoS Pathog., 2011;7(5):e1001338.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010;82(5):968-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009;51(4):734-49.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  15. The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma.
    Authors: Marchesi F, Piemonti L, Fedele G, Destro A, Roncalli M, Albarello L, Doglioni C, Anselmo A, Doni A, Bianchi P, Laghi L, Malesci A, Cervo L, Malosio M, Reni M, Zerbi A, Di Carlo V, Mantovani A, Allavena P
    Cancer Res., 2008;68(21):9060-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 15
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 148  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG1 Isotype Control

Ctrl MAB002R 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4 (Based on 1 review)

Have you used Human Integrin  beta 1/CD29 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human Integrin  beta 1/CD29 Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

Filter by Ratings
Very Good
(1)
All Ratings
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Integrin  beta 1/CD29 Antibody MAB17781 Array MAB17781
Enlarge
Very Good
 WB Human Anonymous 04/24/2018
Show
Image Details
Western Blot Human Integrin  beta 1/CD29 Antibody MAB17781
Western Blot: Human Integrin  beta 1/CD29 Antibody [MAB17781].

Summary

Application Western Blot
Sample Tested blood endothelial cells
Species Human

Other Experimental Details

Other Experimental Details Worked in both reducing and non-reducing conditionsBand seen around 130kDaWould use again on a new blot, not a stripped blot as got very similar band to that with alpha 5

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee