Human Integrin  beta 1/CD29 Antibody

(12 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin beta 1/CD29.
  • Source
    Monoclonal Mouse IgG1 Clone # P5D2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human skin keratinocytes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoprecipitation

    Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
    Joneckis, C.C. et al. (1993) Blood 82:3548.

  • Blockade of Receptor-ligand Interaction
    Mukhopadhyay, N.K. et al. (2004) Ann. Thorac. Surg. 78:450.
    Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
Integrin  beta 1/CD29 in human PBMCs. Integrin  beta 1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.
  • References:
    1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
    2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.
  • Entrez Gene IDs:
    3688 (Human); 16412 (Mouse)
  • Alternate Names:
    CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Fibronectin-guided migration of carcinoma collectives
    Authors: S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg
    Nat Commun, 2017;8(0):14105.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016;6(0):34310.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
    Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
    Cell Commun Signal, 2014;12(0):36.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.
    Authors: Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C
    J Exp Med, 2014;211(13):2549-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  5. The alpha-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in beta3 integrins.
    Authors: Rui X, Mehrbod M, Van Agthoven J, Anand S, Xiong J, Mofrad M, Arnaout M
    J Biol Chem, 2014;289(33):23256-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Cultivation of corneal endothelial cells on a pericellular matrix prepared from human decidua-derived mesenchymal cells.
    Authors: Numata R, Okumura N, Nakahara M, Ueno M, Kinoshita S, Kanematsu D, Kanemura Y, Sasai Y, Koizumi N
    PLoS ONE, 2014;9(2):e88169.
  7. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.
    Authors: Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, Paul A, Esposito E, Gridley S, Leitinger B, Naegle K, Huang P
    Biochem J, 2013;454(3):501-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly.
    Authors: Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM
    PLoS ONE, 2012;7(3):e33915.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection.
    Authors: Shannon-Lowe C, Rowe M
    PLoS Pathog., 2011;7(5):e1001338.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010;82(5):968-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009;51(4):734-49.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  12. The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma.
    Authors: Marchesi F, Piemonti L, Fedele G, Destro A, Roncalli M, Albarello L, Doglioni C, Anselmo A, Doni A, Bianchi P, Laghi L, Malesci A, Cervo L, Malosio M, Reni M, Zerbi A, Di Carlo V, Mantovani A, Allavena P
    Cancer Res., 2008;68(21):9060-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 12 Citations
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