Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IQGAP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IQGAP2
Asn444-Ser633
Accession # Q13576

Specificity

Detects endogenous human IQGAP2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human IQGAP2 Antibody

Detection of Human IQGAP2 antibody by Western Blot.

Detection of Human IQGAP2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP2 at approximately 185 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human IQGAP2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IQGAP2

IQGAP2 (IQ motif with Ras GAP-related domain 2) is a 180‑190 kDa member of the IQGAP family of proteins. It has limited expression (hepatocytes, platelets, parietal cells), binds to GTP-bound Cdc42, blocking GTP hydrolysis, and participates in actin cross-linking. Human IQGAP2 is 1575 amino acids (aa) in length. It contains one actin-binding CH domain (aa 41‑156), a Pro-binding WWP region (aa 594‑627), three consecutive calmodulin-binding IQ motifs (aa 690‑779), and a noncatalytic Ras-GAP domain (aa 917‑1150). There are multiple IQGAP2 splice variants. One shows a seven aa substitution for aa 1‑454 coupled to a deletion of aa 642‑698, while a second shows a 67 aa substitution for aa 303 and 304. IQGAP-2 is 59% aa identical to IQGAP1.

Long Name

IQ Motif Containing GTPase Activating Protein 2

Alternate Names

IQ motif containing GTPase activating protein 2, ras GTPase-activating-like protein IQGAP2

Entrez Gene IDs

10788 (Human); 544963 (Mouse)

Gene Symbol

IQGAP2

UniProt

Q13576

Additional IQGAP2 Products

Product Documents for Human IQGAP2 Antibody

Certificate of Analysis

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Product Specific Notices for Human IQGAP2 Antibody

For research use only

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