Detection of Human IQGAP2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5068) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP2 at approximately 185 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IQGAP2 (IQ motif with Ras GAP-related domain 2) is a 180‑190 kDa member of the IQGAP family of proteins. It has limited expression (hepatocytes, platelets, parietal cells), binds to GTP-bound Cdc42, blocking GTP hydrolysis, and participates in actin cross-linking. Human IQGAP2 is 1575 amino acids (aa) in length. It contains one actin-binding CH domain (aa 41‑156), a Pro-binding WWP region (aa 594‑627), three consecutive calmodulin-binding IQ motifs (aa 690‑779), and a noncatalytic Ras-GAP domain (aa 917‑1150). There are multiple IQGAP2 splice variants. One shows a seven aa substitution for aa 1‑454 coupled to a deletion of aa 642‑698, while a second shows a 67 aa substitution for aa 303 and 304. IQGAP-2 is 59% aa identical to IQGAP1.
IQ Motif Containing GTPase Activating Protein 2
Entrez Gene IDs:
10788 (Human); 544963 (Mouse)
IQ motif containing GTPase activating protein 2; IQGAP2; ras GTPase-activating-like protein IQGAP2
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