Human ISG15/UCRP Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4845R Datasheet / COA / SDS

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AF4845R-100UG

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All ISG15/UCRP Antibodies

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Human ISG15/UCRP Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human ISG15 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ISG15
Gly2-Gly157
Accession # ABW03539

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ISG15/UCRP

ISG15 (interferon-induced 15 kDa protein; also IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation, instead, it is part of the anti‑virus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2‑78 and 79‑157) and a propeptide (aa 15‑165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.

Long Name

ISG15 Ubiquitin-like Modifier

Entrez Gene IDs

9636 (Human); 100038882 (Mouse); 298693 (Rat)

Alternate Names

G1P2; G1P2interferon-induced 17-kDa/15-kDa protein; IFI15; IFI15hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein, 15 kDa; IP17; ISG15 ubiquitin-like modifier; ISG15; Ubiquitin cross-reactive protein; ubiquitin-like protein ISG15; UCRP; UCRPIP17