Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ISG15/UCRP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ISG15
Gly2-Gly157
Accession # ABW03539

Specificity

Detects endogenous human ISG15 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ISG15/UCRP Antibody

Detection of Human ISG15/UCRP antibody by Western Blot.

Detection of Human ISG15/UCRP by Western Blot.

Western blot shows lysates of DLD-1 clone 2C2 human colon adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and CCD-1070Sk human foreskin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human ISG15/UCRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4845) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human ISG15/UCRP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: DLD-1 clone 2C2 human colon adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and CCD-1070Sk human foreskin fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ISG15/UCRP

ISG15 (interferon-induced 15 kDa protein; also IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation, instead, it is part of the anti‑virus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2‑78 and 79‑157) and a propeptide (aa 15‑165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.

Long Name

ISG15 Ubiquitin-like Modifier

Alternate Names

G1P2, IFI15, IP17, UCRP

Entrez Gene IDs

9636 (Human); 100038882 (Mouse); 298693 (Rat)

Gene Symbol

ISG15

UniProt

ABW03539

Additional ISG15/UCRP Products

Product Documents for Human ISG15/UCRP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ISG15/UCRP Antibody

For research use only

Citations for Human ISG15/UCRP Antibody

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