Human JAM-A APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1103A
Detection of JAM‑A in MCF‑7 Human Cell Line by Flow Cytometry.
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Human JAM-A APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human JAM-A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) JAM-B, recombinant mouse (rm) JAM-A, or rmJAM-4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 654806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-A
Ser28-Ala242 (predicted)
Accession # Q9Y624
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of JAM‑A antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of JAM‑A in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human JAM-A APC-conjugated Monoclonal Antibody (Catalog # FAB1103A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: JAM-A

The family of Junctional Adhesion Molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as Platelet Adhesion Molecule 1 (PAM-1) and Platelet F11 Receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1 - 4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig-like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interaction also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta 2 Integrin Lymphocyte Function-associated Antigen-1 (LFA-1). This JAM-A-LFA-1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind Reovirus Attachment Protein Sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Sobocka, M.B. et al. (2000) Blood 95:2600.
  4. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
  5. Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.
  6. Ostermann, G. et al. (2002) Nature Immunol. 3:151.
  7. Barton, E.S. et al. (2001) Cell 104:441.
Long Name
Junctional Adhesion Molecule A
Entrez Gene IDs
50848 (Human); 16456 (Mouse); 101925695 (Cynomolgus Monkey)
Alternate Names
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1

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