Human JAM-A Antibody

Catalog # Availability Size / Price Qty
MAB1103
MAB1103-SP

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Detection of JAM‑A in MCF‑7 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Reviews (1)

Human JAM-A Antibody Summary

Species Reactivity
Human
Specificity
Detects human JAM-A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) JAM-B, recombinant mouse (rm) JAM-A, or rmJAM-4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 654806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-A
Ser28-Ala242 (predicted)
Accession # Q9Y624
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of JAM-A antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of JAM‑A in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Human JAM-A Monoclonal Antibody (Catalog # MAB1103, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Immunocytochemistry JAM-A antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

JAM‑A in MCF‑7 Human Cell Line. JAM-A was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human JAM-A Monoclonal Antibody (Catalog # MAB1103) at 20 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to intercellular junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry JAM-A antibody in Human Endometrial Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

JAM-A in Human Endometrial Cancer Tissue. JAM-A was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Human JAM-A Monoclonal Antibody (Catalog # MAB1103) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-A

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1 - 4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig-like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta 2 integrin lymphocyte function-associated antigen-1 (LFA-1). This JAM-A-LFA-1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Sobocka, M.B. et al. (2000) Blood 95:2600.
  4. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
  5. Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.
  6. Ostermann, G. et al. (2002) Nature Immunol. 3:151.
  7. Barton, E.S. et al. (2001) Cell 104:441.
Long Name
Junctional Adhesion Molecule A
Entrez Gene IDs
50848 (Human); 16456 (Mouse); 101925695 (Cynomolgus Monkey)
Alternate Names
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1

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Citation for Human JAM-A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-18 Is Involved in Eosinophil-Mediated Tumoricidal Activity against a Colon Carcinoma Cell Line by Upregulating LFA-1 and ICAM-1.
    Authors: Gatault S, Delbeke M, Driss V, Sarazin A, Dendooven A, Kahn J, Lefevre G, Capron M
    J Immunol, 2015;195(5):2483-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Human JAM-A Antibody
By Anonymous on 07/25/2022
Application: IHC Sample Tested: Kidney tissue Species: Human