Human KCC4/SLC12A7 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB9030R-025
Detection  of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7  and eGFP by Flow Cytometry.
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Human KCC4/SLC12A7 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLC12A7 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 891526
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human SLC12A7
Met1-Ser1083
Accession # Q9Y666
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7  and eGFP by Flow Cytometry. View Larger

Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SLC12A7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human KCC4/SLC12A7 Alexa Fluor® 647‑conjugated Monoclonal Antibody(Catalog # FAB9030R). Quadrant markers were set based on control antibody staining (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KCC4/SLC12A7

KCC4 (Potassium Chloride Cotransporter 4), also known as SLC12A7, is a 130-145 kDa member of the SLC12A family of ion transporter molecules. It is a 12-transmembrane, 1083 amino acid (aa), variably glycosylated protein that basically serves three functions: one, it acts as a membrane-bound scaffold for Ezrin-associated cytoskeletal organization; two, it is involved in salt resorption; and three, while inactive under isotonic conditions, it is activated during cell swelling, reducing intracellular ion concentration with a subsequent reduction in cell volume. On the cell surface, KCC4 presents as either a homooligomer, or a heterooligomer in a complex with KCC2 or NKCC1. Cell known to express KCC4 include PNS neurons, select CNS neurons, choroid plexus epithelium, proximal convoluted tubule and thick ascending loop of Henle epithelium, pancreatic islet alpha -cells, gastric parietal cells, alpha -intercalated cells of the renal collecting duct, and erythrocytes. The extracellular domains of human mouse KCC4 share 85% aa sequence identity.

Long Name
Potassium/Chloride Transporter KCC4
Entrez Gene IDs
10723 (Human); 20499 (Mouse); 308069 (Rat)
Alternate Names
DKFZp434F076; KCC4; KCC4Electroneutral potassium-chloride cotransporter 4; K-Cl cotransporter 4; potassium/chloride transporter KCC4; SLC12A7; solute carrier family 12 (potassium/chloride transporters), member 7; solute carrier family 12 member 7

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