|Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SLC12A7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human KCC4/SLC12A7 Alexa Fluor® 647‑conjugated Monoclonal Antibody(Catalog # FAB9030R). Quadrant markers were set based on control antibody staining (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.|
KCC4 (Potassium Chloride Cotransporter 4), also known as SLC12A7, is a 130-145 kDa member of the SLC12A family of ion transporter molecules. It is a 12-transmembrane, 1083 amino acid (aa), variably glycosylated protein that basically serves three functions: one, it acts as a membrane-bound scaffold for Ezrin-associated cytoskeletal organization; two, it is involved in salt resorption; and three, while inactive under isotonic conditions, it is activated during cell swelling, reducing intracellular ion concentration with a subsequent reduction in cell volume. On the cell surface, KCC4 presents as either a homooligomer, or a heterooligomer in a complex with KCC2 or NKCC1. Cell known to express KCC4 include PNS neurons, select CNS neurons, choroid plexus epithelium, proximal convoluted tubule and thick ascending loop of Henle epithelium, pancreatic islet alpha -cells, gastric parietal cells, alpha -intercalated cells of the renal collecting duct, and erythrocytes. The extracellular domains of human mouse KCC4 share 85% aa sequence identity.