Human KCC4/SLC12A7 Antibody
Human KCC4/SLC12A7 Antibody Summary
Accession # Q9Y666
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SLC12A7 or (B) human MRGPRF and eGFP was stained with Mouse Anti-Human KCC4/SLC12A7 Monoclonal Antibody (Catalog # MAB9030) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cation chloride cotransporters, including the potassium-chloride cotransporters (KCCs), are involved in the electroneutral movement of ions across the plasma membrane. SLC12A7 contains 12 membrane-spanning segments, 8 phosphorylation sites, 7 of which are in the C terminus, and 4 potential N-glycosylation sites. It is important for potassium recycling by siphoning potassium ions after their exit from outer hair cells into supporting Deiters cells, where potassium enters the gap junction pathway.
Citation for Human KCC4/SLC12A7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Surfaceome Profiling of Cell Lines and Patient-Derived Xenografts Confirm FGFR4, NCAM1, CD276, and Highlight AGRL2, JAM3, and L1CAM as Surface Targets for Rhabdomyosarcoma
Authors: A Timpanaro, C Piccand, AC Uldry, PK Bode, D Dzhumashev, R Sala, M Heller, J Rössler, M Bernasconi
International Journal of Molecular Sciences, 2023-01-30;24(3):.
Sample Types: Whole Cells
Applications: Flow Cytometry
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