Human KCC4/SLC12A7 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SLC12A7 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 891526
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with  human SLC12A7
    Met1-Ser1083
    Accession # Q9Y666
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of KCC4/SLC12A7 in HEK293 Human Cell Line Transfected with Human SLC12A7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SLC12A7 or (B) human MRGPRF and eGFP was stained with Mouse Anti-Human KCC4/SLC12A7 Monoclonal Antibody (Catalog # MAB9030) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # F0101B).  Quadrant markers were set based on control antibody staining (Catalog # MAB002).  View our protocol for Staining Membrane-associated Proteins.   
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KCC4/SLC12A7
Cation chloride cotransporters, including the potassium-chloride cotransporters (KCCs), are involved in the electroneutral movement of ions across the plasma membrane. SLC12A7 contains 12 membrane-spanning segments, 8 phosphorylation sites, 7 of which are in the C terminus, and 4 potential N-glycosylation sites. It is important for potassium recycling by siphoning potassium ions after their exit from outer hair cells into supporting Deiters cells, where potassium enters the gap junction pathway.
  • Long Name:
    Potassium/Chloride Transporter KCC4
  • Entrez Gene IDs:
    10723 (Human); 20499 (Mouse); 308069 (Rat)
  • Alternate Names:
    DKFZp434F076; KCC4/SLC12A7; KCC4Electroneutral potassium-chloride cotransporter 4; K-Cl cotransporter 4; potassium/chloride transporter KCC4; SLC12A7; solute carrier family 12 (potassium/chloride transporters), member 7; solute carrier family 12 member 7
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 99  
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Mouse IgG1 Isotype Control

Ctrl MAB002R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 16  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NL557 Affinity Purified PAb

Flow, IHC, ICC NL007 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
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