Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8920R-025
Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with KIR2DS5/CD158g by Flow Cytometry.
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Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DS5/CD158g in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1165A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human KIR2DS5/CD158g
His22-His245
Accession # Q14953
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DS5/CD158g antibody in BaF3 Mouse Cell Line Transfected with KIR2DS5/CD158g antibody by Flow Cytometry. View Larger

Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with KIR2DS5/CD158g by Flow Cytometry. BaF3 mouse pro-B cell line transfected with KIR2DS5/CD158g were stained with Rabbit Anti-Human KIR2DS5/CD158g Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8920R, filled histogram) or control antibody Rabbit Anti-Human KIR3DL3/CD158z Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8919R, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS5/CD158g

Killer-immunoglobulin-like receptors (KIR) are polymorphous activating and inhibitory receptors expressed on the surface of NK cells and some T cells. KIR genes are highly homologous. KIR proteins expressing the long (L) cytoplasmic domain are inhibitory, while KIRs with short (S) cytoplasmic domains are activating. Thus, KIR2DS5 is a 65 kDa type I transmembrane activating receptor for NK cells, and is thought to play a role in NK cell function in response to a number of conditions. While many KIR proteins bind HLA-class I molecules, the ligand(s) for KIR2DS5 remain unclear.  

Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 5
Entrez Gene IDs
3810 (Human)
Alternate Names
killer cell immunoglobulin-like receptor 2DS5; CD158g; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; KIR2DS5; NKAT9

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

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Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody
By Anonymous on 11/02/2017
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Sample was known to have the gene but the antibody failed to stain for it.

Technical Service will be following up regarding this concern