Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody.
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human KIR2DS5/CD158g in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1165A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human KIR2DS5/CD158g
    His22-His245
    Accession # Q14953
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with KIR2DS5/CD158g by Flow Cytometry. BaF3 mouse pro-B cell line transfected with KIR2DS5/CD158g were stained with Rabbit Anti-Human KIR2DS5/CD158g Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8920R, filled histogram) or control antibody Rabbit Anti-Human KIR3DL3/CD158z Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8919R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KIR2DS5/CD158g
Killer-immunoglobulin-like receptors (KIR) are polymorphous activating and inhibitory receptors expressed on the surface of NK cells and some T cells. KIR genes are highly homologous. KIR proteins expressing the long (L) cytoplasmic domain are inhibitory, while KIRs with short (S) cytoplasmic domains are activating. Thus, KIR2DS5 is a 65 kDa type I transmembrane activating receptor for NK cells, and is thought to play a role in NK cell function in response to a number of conditions. While many KIR proteins bind HLA-class I molecules, the ligand(s) for KIR2DS5 remain unclear.  
  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 5
  • Entrez Gene IDs:
    3810 (Human)
  • Alternate Names:
    killer cell immunoglobulin-like receptor 2DS5; CD158g; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; KIR2DS5; NKAT9
Related Research Areas
Isotype Controls
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Rabbit IgG Alexa Fluor® 647-conjugated Antibody

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Average Rating: 1 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody FAB8920R
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 Flow Human Anonymous 11/02/2017
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Flow Cytometry Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody FAB8920R
Flow Cytometry: Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody [FAB8920R]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSample was known to have the gene but the antibody failed to stain for it. Technical Service will be following up regarding this concern

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