Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8920R-025
Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with KIR2DS5/CD158g by Flow Cytometry.
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Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DS5/CD158g in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1165A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human KIR2DS5/CD158g
His22-His245
Accession # Q14953
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with KIR2DS5/CD158g by Flow Cytometry. View Larger

Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with KIR2DS5/CD158g by Flow Cytometry. BaF3 mouse pro-B cell line transfected with KIR2DS5/CD158g were stained with Rabbit Anti-Human KIR2DS5/CD158g Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8920R, filled histogram) or control antibody Rabbit Anti-Human KIR3DL3/CD158z Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8919R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS5/CD158g

Killer-immunoglobulin-like receptors (KIR) are polymorphous activating and inhibitory receptors expressed on the surface of NK cells and some T cells. KIR genes are highly homologous. KIR proteins expressing the long (L) cytoplasmic domain are inhibitory, while KIRs with short (S) cytoplasmic domains are activating. Thus, KIR2DS5 is a 65 kDa type I transmembrane activating receptor for NK cells, and is thought to play a role in NK cell function in response to a number of conditions. While many KIR proteins bind HLA-class I molecules, the ligand(s) for KIR2DS5 remain unclear.  

Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 5
Entrez Gene IDs
3810 (Human)
Alternate Names
killer cell immunoglobulin-like receptor 2DS5; CD158g; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; KIR2DS5; NKAT9

Product Datasheets

FAQs

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Reviews for Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody

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Human KIR2DS5/CD158g Alexa Fluor® 647-conjugated Antibody
By Anonymous on 11/02/2017
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Sample was known to have the gene but the antibody failed to stain for it. Technical Service will be following up regarding this concern